Active • Host: Insect cells • AA: 808-1,132 • Tag: N-terminal His • MW: 42.5 kDa
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JAK2 (human, recombinant)

Item No. 33742

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Janus-Associated Kinase 2
  • JTK10
  • Tyrosine-protein Kinase JAK2
Purity
≥71% estimated by SDS-PAGE
Source
Active recombinant human N-terminal His-tagged JAK2 expressed in insect cells
Amino Acids
808-1,132
MW
42.5 kDa
Formulation (Request formulation change)
Lyophilized from sterile 40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, 3 mM DTT, 0.04% Tween 20, and 20% glycerol
UniProt Accession №
O60674
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    20 µg

    $455

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    Product Description

    JAK2 is a non-receptor tyrosine kinase that has roles in immune signaling.1,2 It is composed of N-terminal FERM and SH2 domains, a regulatory pseudokinase domain, and a C-terminal kinase domain.1 It is widely expressed and associates with class I and class II cytokine receptors at the plasma membrane.3,4 Activation of these cytokine receptors activates JAK2 and induces its dimerization and kinase activity, leading to JAK2 phosphorylation of STAT transcription factors and transcription of immune-related target genes. JAK2 signaling is inhibited by the suppressor of cytokine signaling (SOCS) proteins SOCS-1 and SOCS-3.5,6 Gain-of-function mutations in JAK2 are associated with various blood disorders, including leukemias and myeloproliferative neoplasms.4 Cayman’s JAK2 (human, recombinant) protein can be used for enzyme activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Leonard, W.J., and O'Shea, J.J. Jaks and STATs: Biological implications. Annu. Rev. Immunol. 16, 293-322 (1998).

    2. Haan, C., Kreis, S., Margue, C., et al. Jaks and cytokine receptors—an intimate relationship. Biochem. Pharmacol. 72(11), 1538-1546 (2006).

    3. Parganas, E., Wang, D., Stravopodis, D., et al. Jak2 is essential for signaling through a variety of cytokine receptors. Cell 93(3), 385-395 (1998).

    4. Hubbard, S.R. Mechanistic insights into regulation of JAK2 tyrosine kinase. Front. Endocrinol. (Lausanne) 8, 361 (2018).

    5. Kile, B.T., and Alexander, W.S. The suppressors of cytokine signalling (SOCS). Cell. Mol. Life Sci. 58(11), 1627-1635 (2001).

    6. Yoshimura, A., and Yasukawa, H. JAK’s SOCS: A mechanism of inhibition. Immunity 36(2), 157-159 (2012).