For immunochemical detection of citrullinated myelin basic protein
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Citrullinated Myelin Basic Protein (R122) Monoclonal Antibody (Clone 1H1)

Item No. 33910

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • citMBP
  • Citrullinated Myelin A1 Protein
  • Citrullinated Myelin Membrane Encephalitogenic Protein
  • Myelin A1 Protein
  • Myelin Membrane Encephalitogenic Protein
Immunogen
Peptide from the C-terminal region of human myelin basic protein (isoform 5) with citrulline at R122
Clone Designation
1H1
MW
12-18 kDa
Formulation (Request formulation change)
25 µg of protein G-purified monoclonal antibody
Storage Buffer
0.1 M Tris-glycine, pH 7.4, with 150 mM sodium chloride and 0.05% sodium azide
Host
Mouse
Isotype
IgG1
Applications
ELISA, IHC, WB
Cross Reactivity
(+) Citrullinated myelin basic protein (R122)(-) Native myelin basic protein
Species Reactivity
(+) Human(+) Mouse
UniProt Accession №
P02686
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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    25 µg

    $192

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    Product Description

    Citrullinated myelin basic protein (citMBP) is a citrullinated form of MBP, a protein that is integral to myelin stability in the CNS.1 MBP contains 19 arginine residues that can be citrullinated by peptidylarginine deiminases (PADs), decreasing the positive charge of MBP.1,2 The loss of positive charge disrupts MBP-lipid interactions, leading to myelin destabilization and loss and increases its susceptibility for degradation. MBP is degraded by the myelin-associated protease cathepsin D, which leads to exposure and release of immunodominant epitopes.1,3 The percentage of MBP that is citrullinated is increased in the postmortem brain from patients with multiple sclerosis (MS), with an even greater percentage citrullinated in Marburg disease, a more severe form of MS.1 citMBP isolated from patients with multiple sclerosis (MS) is degraded by cathepsin D at a higher rate than MBP isolated from healthy controls.1,3 Cayman’s Citrullinated Myelin Basic Protein (R122) Monoclonal Antibody (Clone 1H1) can be used for ELISA, immunohistochemistry (IHC), and Western blot applications. The antibody recognizes citMBP from human and mouse samples and was generated using a peptide immunogen containing citrulline at R122 (human myelin basic protein sequence, isoform 5).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Yang, L., Tan, D., and Piao, H. Myelin basic protein citrullination in multiple sclerosis: A potential therapeutic target for the pathology. Neurochem. Res. 41(8), 1845-1856 (2016).

    2. Mastronardi, F.G., and Moscarello, M.A. Molecules affecting myelin stability: A novel hypothesis regarding the pathogenesis of multiple sclerosis. J. Neurosci. Res. 80(3), 301-308 (2005).

    3. Cao, L., Goodin, R., Wood, D., et al. Rapid release and unusual stability of immunodominant peptide 45-89 from citrullinated myelin basic protein. Biochemistry 38(19), 6157-6163 (1999).