Active • Host: Insect cells • AA: 1,362-2,549 (mTOR), 2-326 (mLST8), and 2-1,335 (Raptor) • Tag: N-terminal DYKDDDDK on mTOR, N-terminal His on mLST8, N-terminal His on Raptor • MW: 137 (mTOR), 37 (mLST8), and 150 (Raptor) kDa
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mTOR/mLST8/Raptor Complex (human, recombinant)

Item No. 33985

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • mTOR:
  • FKBP12-Rapamycin Complex-associated Protein
  • FK506-binding Protein 12-Rapamycin Complex-associated Protein 1
  • Mammalian Target of Rapamycin
  • Mechanistic Target of Rapamycin
  • Rapamycin and FKBP12 Target 1
  • Rapamycin Target Protein 1
  • Serine/Threonine-protein Kinase mTOR
  • mLST8:
  • G protein β Subunit-like
  • Gable
  • GβL
  • Target of Rapamycin Complex Subunit LST8
  • TORC Subunit LST8
  • Mammalian Lethal with SEC13 Protein 8
  • Raptor:
  • Regulatory-associated Protein of mTOR
  • RPTOR
  • p150 Target of Rapamycin-scaffold Protein
  • p150 TOR-scaffold Protein
Purity
≥50% estimated by SDS-PAGE
Source
Active recombinant human N-terminal DYKDDDDK-tagged mTOR, N-terminal His-tagged mLST8, and N-terminal His-tagged Raptor expressed in insect cells
Amino Acids
1,362-2,549 (mTOR), 2-326 (mLST8), and 2-1,335 (Raptor)
MW
137 (mTOR), 37 (mLST8), and 150 (Raptor) kDa
Formulation (Request formulation change)
40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, 20% glycerol, and variable FLAG peptide
Host
Insect cells
UniProt Accession №
P42345 (mTOR), Q9BVC4 (mLST8), Q8N122 (Raptor)
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    10 µg

    $539

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    Cayman Chemical
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    Product Description

    mTORC1 is a protein complex composed of the serine/threonine kinase mammalian target of rapamycin (mTOR), TOR complex subunit LST8 (mLST8), regulatory-associated protein of mTOR (Raptor), proline-rich Akt substrate 40 kDa (PRAS40), and DEP domain-containing mTOR-interacting protein (Deptor) that functions as a nutrient sensor and regulates cell growth and metabolism.1,2,3 PRAS40 and Deptor act as negative regulators of mTORC1 activity, and mLST8 and Raptor as scaffold proteins for substrates such as 4E-BP1 and p70 ribosomal S6 kinase 1 (p70S6K1). mTORC1 localizes to the plasma membrane, lysosome, and nucleus depending on its upstream activation signal, which include AMPK, PI3K, and Akt.4,1 Activation of mTORC1 is influenced by growth factors, energy status, oxygen levels, and amino acid availability and is involved in a wide variety of processes, including aging, autophagy, stem cell and immune function, cellular senescence, and protein translation.1,5,6,3 Dysregulation of the mTOR signaling pathway is associated with various pathologies, including cancer, rheumatoid arthritis, epilepsy, neurodegenerative diseases, and diabetes.1,7,3 Cayman’s mTOR/mLST8/Raptor Complex (human, recombinant) protein can be used for enzyme activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Laplante, M., and Sabatini, D.M. mTOR signaling at a glance. J. Cell Sci. 122(pt 20), 3589-3594 (2009).

    2. Zarogoulidis, P., Lampaki, S., Turner, J.F., et al. mTOR pathway: A current, up-to-date mini-review (review). Oncol. Lett. 8(6), 2367-2370 (2014).

    3. Showkat, M., Beigh, M.A., and Andrabi, K.I. mTOR signaling in protein translation regulation: Implications in cancer genesis and therapeutic interventions. Mol. Biol. Int. 686984 (2014).

    4. Zhou, X., Clister, T., Lowry, P.R., et al. Dynamic visualization of mTORC1 activity in living cells. Cell Rep. 10(10), 1767-1777 (2015).

    5. Weichhart, T. mTOR as regulator of lifespan, aging, and cellular senescence: A mini-review. Gerontology 64(2), 127-134 (2018).

    6. Kim, Y.C., and Guan, K.-L. mTOR: A pharmacological target for autophagy regulation. J. Clin. Invest. 125(1), 25-32 (2015).

    7. Wong, M. A critical review of mTOR inhibitors and epilepsy: From basic science to clinical trials. Expert Rev. Neurother. 13(6), 657-669 (2013).