Active • Host: HEK293 cells • AA: 33-531 • Tag: C-terminal His • MW: 57.6 kDa
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PDGFRβ/CD140b Extracellular Domain (human, recombinant)

Item No. 33986

Product Insert (PDF)
Technical Information
Synonyms
  • CD140 Antigen-like Family Member B
  • PDGFR-1
  • Platelet-derived Growth Factor Receptor 1
  • Platelet-derived Growth Factor Receptor β
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/µg determined by the LAL endotoxin assay
Source
Active recombinant human C-terminal His-tagged PDGFRβ expressed in HEK293 cells
Amino Acids
33-531
MW
57.6 kDa
Formulation (Request formulation change)
Lyophilized from sterile PBS, pH 7.4, with 5% Trehalose, 5% Mannitol, and 0.01% Tween-80
UniProt Accession №
P09619
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    100 µg

    $301

    Add

    1 mg

    $1,952

    Add
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    Product Description

    PDGFRβ, also known as CD140b, is a type I transmembrane glycoprotein and receptor tyrosine kinase encoded by PDGFRB in humans.1 It is composed of five extracellular immunoglobulin-like (Ig-like) domains, a transmembrane domain, and an intracellular kinase domain. PDGFRβ is ubiquitously expressed and functions as a homodimer or a heterodimer with PDGFRα. Binding of the growth factor PDGF-BB or PDGF-DD to PDGFRβ promotes homodimerization, induces receptor autophosphorylation and a conformational change that facilitates access to the intracellular kinase domain, and intracellular signaling through multiple pathways, including ERK/MAPK, PI3K/AKT/mTOR, PLC/PKC, and JAK/STAT.1,2,3 Knockdown of Pdgfrb decreases the number of vascular smooth muscle cells, induces CNS-specific reduction in pericytes, and is postnatally lethal in mice. De novo mutations in PDGFRB have been found in patients with novel overgrowth syndrome.4 Cayman's PDGFRβ/CD140b Extracellular Domain (human, recombinant) protein can be used for binding assays. This protein consists of 510 amino acids, has a calculated molecular weight of 57.6 kDa, and a predicted N-terminus of Leu33 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is approximately 95 to 100 kDa due to glycosylation.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Heldin, C.-H., and Lennartsson, J. Structural and functional properties of platelet-derived growth factor and stem cell factor receptors. Cold Spring Harb. Perspect. Biol. 5(8), a009100 (2013).

    2. Kelly, J.D., Haldeman, B.A., Grant, F.J., et al. Platelet-derived growth factor (PDGF) stimulates PDGF receptor subunit dimerization and intersubunit trans-phosphorylation. The Journal of Biological Chemisty 266(14), 8987-8992 (1991).

    3. Olson, L.E., and Soriano, P. PDGFRβ signaling regulates mural cell plasticity and inhibits fat development. Dev. Cell 20(6), 815-826 (2011).

    4. Takenouchi, T., Yamaguchi, Y., Tanikawa, A., et al. Novel overgrowth syndrome phenotype due to recurrent de novo PDGFRB mutation. J. Pediatr. 166(2), 483-486 (2015).