A fluorogenic substrate for leucine aminopeptidase
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Unlabeled Version(s)
34342L-Leucine
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L-Leu-AMC (hydrochloride)

Item No. 34267

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(2S)-2-amino-4-methyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-pentanamide, monohydrochloride
CAS Number
62480-44-8
Synonyms
  • Leu-MCA
  • L-Leucine-7-amido-4-methylcoumarin
  • 7-L-Leucyl-4-methylcoumarinylamide
Molecular Formula
C16H20N2O3 • HCl
Formula Weight
324.8
Purity
≥98%
Emission
440-460 nm
Excitation
340-360 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 25 mg/mlDMSO: 25 mg/mlDMSO:PBS (pH 7.2) (1:3): 0.25 mg/ml
λmax
214, 296, 326 nm
SMILES
CC(C1=CC=C(NC([C@@H](N)CC(C)C)=O)C=C1O2)=CC2=O.Cl
InChi Code
InChI=1S/C16H20N2O3.ClH/c1-9(2)6-13(17)16(20)18-11-4-5-12-10(3)7-15(19)21-14(12)8-11;/h4-5,7-9,13H,6,17H2,1-3H3,(H,18,20);1H/t13-;/m0./s1
InChi Key
VCRXITKKWBOQRZ-ZOWNYOTGSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    50 mg

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    100 mg

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    250 mg

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    500 mg

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    Product Description

    L-Leu-AMC is a fluorogenic substrate for leucine aminopeptidase.1 Upon enzymatic cleavage by leucine aminopeptidase, AMC is released and its fluorescence can be used to quantify leucine aminopeptidase activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Izquierdo, M., Lin, D., O'Neill, S., et al. Development of a high-throughput screening assay to identify inhibitors of the major M17-leucyl aminopeptidase from Trypanosoma cruzi using rapidfire mass spectrometry. SLAS Discov. 25(9), 1064-1071 (2020).