A fluorogenic substrate for CYPs
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7-Methoxy-4-(trifluoromethyl)coumarin

Item No. 34398

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-methoxy-4-(trifluoromethyl)-2H-1-benzopyran-2-one
CAS Number
575-04-2
Synonyms
  • MFC
Molecular Formula
C11H7F3O3
Formula Weight
244.2
Purity
≥95%
Emission
510 nm
Excitation
410 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMF:PBS (pH 7.2) (1:2): 0.3 mg/mlDMSO: 25 mg/mlEthanol: 10 mg/ml
λmax
334 nm
SMILES
O=C1OC2=C(C(C(F)(F)F)=C1)C=CC(OC)=C2
InChi Code
InChI=1S/C11H7F3O3/c1-16-6-2-3-7-8(11(12,13)14)5-10(15)17-9(7)4-6/h2-5H,1H3
InChi Key
HAZHUELNIGDYQH-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    50 mg

    $230

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    100 mg

    $437

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    250 mg

    $977

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    Product Description

    7-Methoxy-4-(trifluoromethyl)coumarin is a fluorogenic substrate for cytochrome P450s (CYPs).1 Upon enzymatic cleavage by CYPs, 7-hydroxy-4-(trifluoromethyl)coumarin (HFC) is released and its fluorescence can be used to quantify CYP activity. HFC displays excitation/emission maxima of 410/510 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Donato, M.T., Jiménez, N., Castell, J.V., et al. Fluorescence-based assays for screening nine cytochrome P450 (P450) activities in intact cells expressing individual human P450 enzymes. Drug Metab. Dispos. 32(7), 699-706 (2004).