A fluorescent dye
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Isomer(s)
290466-Carboxytetramethylrhodamine
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5-Carboxytetramethylrhodamine

Item No. 34598

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
9-(2,4-dicarboxyphenyl)-3,6-bis(dimethylamino)-xanthylium, inner salt
CAS Number
91809-66-4
Synonyms
  • 5-TAMRA
Molecular Formula
C25H22N2O5
Formula Weight
430.5
Purity
≥98%
Emission
580 nm
Excitation
546 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 1 mg/mlPBS (pH 7.2): 1 mg/ml
λmax
550 nm
SMILES
O=C(C1=C(C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)C=CC(C(O)=O)=C1)[O-]
InChi Code
InChI=1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)
InChi Key
YMZMTOFQCVHHFB-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $54

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    10 mg

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    25 mg

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    50 mg

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    Product Description

    5-Carboxytetramethylrhodamine is a fluorescent dye that has commonly been used for the covalent labeling of oligonucleotides for DNA analysis.1 It displays excitation and emission maxima of 546 and 580 nm, respectively.2 5-Carboxytetramethylrhodamine has also been used in various fluorescence polarization assays and protein FRET experiments.2,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kvach, M.V., Stepanova, I.A., Prokhorenko, I.A., et al. Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probes. Bioconjug. Chem. 20(8), 1673-1682 (2009).

    2. Qi, J., Oppenheimer, M., and Sobrado, P. Fluorescence polarization binding assay for Aspergillus fumigatus virulence factor UDP-galactopyranose mutase. Enzyme Res. 513905 (2011).

    3. Casiraghi, A., Longhena, F., Straniero, V., et al. Design and synthesis of fluorescent-methylphenidate analogues for FRET-based assay of synapsin III binding. ChemMedChem 15(14), 1330-1337 (2020).