A fluorogenic substrate for CYPs
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7-Ethoxy-4-(trifluoromethyl)coumarin

Item No. 34610

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-ethoxy-4-(trifluoromethyl)-2H-1-benzopyran-2-one
CAS Number
115453-82-2
Synonyms
  • EFC
Molecular Formula
C12H9F3O3
Formula Weight
258.2
Purity
≥95%
Emission
510 nm
Excitation
410 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Chloroform: slightly solubleMethanol: slightly soluble
SMILES
O=C1OC2=C(C(C(F)(F)F)=C1)C=CC(OCC)=C2
InChi Code
InChI=1S/C12H9F3O3/c1-2-17-7-3-4-8-9(12(13,14)15)6-11(16)18-10(8)5-7/h3-6H,2H2,1H3
InChi Key
OLHOIERZAZMHGK-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    5 mg

    $38

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    10 mg

    $61

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    25 mg

    $123

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    50 mg

    $168

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    Product Description

    7-Ethoxy-5-(trifluoromethyl)coumarin is a fluorogenic substrate for cytochrome P450s (CYPs).1 Upon O-deethylation by CYPs, 7-hydroxy-4-(trifluoromethyl)coumarin (HFC) is released and its fluorescence can be used to quantify CYP activity. HFC displays excitation/emission maxima of 410/510 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Buters, J.T.M., Schiller, C.D., and Chou, R.C. A highly sensitive tool for the assay of cytochrome P450 enzyme activity in rat, dog and man. Direct fluorescence monitoring of the deethylation of 7-ethoxy-4-trifluoromethylcoumarin. Biochem. Pharmacol. 46(9), 1577-1584 (1993).