Active • Host: Insect cells • AA: 2-521 (full length) • Tag: N-terminal His • MW: 60.8 kDa
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Calcineurin A α Isoform (human, recombinant)

Item No. 34630

Product Insert (PDF)
Technical Information
Synonyms
  • Calmodulin-dependent Calcineurin A Subunit α Isoform
  • CALNA1
  • CAM-PRP Catalytic Subunit
  • CCN1
  • CNA1
  • PPP2B
  • PPP3CA
  • Protein Phosphatase 2B
  • Serine/threonine-Protein Phosphatase 2B Catalytic Subunit α Isoform
Purity
≥94% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/µg determined by the LAL endotoxin assay
Source
Active recombinant human N-terminal His-tagged calcineurin A expressed in insect cells
Amino Acids
2-521 (full length)
MW
60.8 kDa
Formulation (Request formulation change)
Lyophilized from sterile 20 mM Tris, pH 8.0, with 500 mM sodium chloride and 10% glycerol
UniProt Accession №
Q08209
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    50 µg

    $361

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    Product Description

    Calcineurin A is the catalytic subunit of calcineurin, a serine/threonine phosphatase with roles in T cell activation, cardiac function, learning and memory, apoptosis, and cell cycle regulation.1,2 Calcineurin A has three isoforms, α, β, and γ, which are encoded by PPP3CA, PPP3CB, and PPP3CC, respectively. Calcineurin A α is composed of an N-terminal catalytical domain, binding regions for the regulatory subunit calcineurin B (Item No. 34631) and the binding partner calmodulin, and a C-terminal autoinhibitory domain.3,4 It is ubiquitously expressed and associates with calcineurin B and calmodulin in a calcium-dependent manner.5,6 Calcineurin A α has roles in kidney development and function, as well as Tau dephosphorylation, but is dispensable for T cell development.6 Calcineurin A α-null mice exhibit impaired kidney function, failure to thrive, and shortened lifespan. Brain calcineurin A α levels are inversely correlated with disease severity in patients with Alzheimer's disease.7 Cayman's Calcineurin A α Isoform (human, recombinant) protein can be used for binding assays. This protein consists of 538 amino acids and has a calculated molecular weight of 60.8 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Rusnak, F., and Mertz, P. Calcineurin: Form and function. Physiol. Rev. 80(4), 1483-1521 (2020).

    2. Mansuy, I. Calcineurin. xPharm: The Comprehensive Pharmacology Reference (2007).

    3. Gooch, J.L. An emerging role for calcineurin Aα in the development and function of the kidney. Am. J. Physiol. Renal. Physiol. 290(4), F769-F776 (2006).

    4. Li, H., Rao, A., and Hogan, P.G. Interaction of calcineurin with substrates and targeting proteins. Trends Cell Biol. 21(2), 91-103 (2011).

    5. Nolze, A., Köhler, C., Ruhs, S., et al. Calcineurin (PPP3CB) regulates angiotensin II-dependent vascular remodelling by potentiating EGFR signalling in mice. Acta Physiol. (Oxf.) e13715 (2021).

    6. Williams, C.R., and Gooch, J.L. Calcineurin inhibitors and immunosuppression - a tale of two isoforms. Expert Rev. Mol. Med. 14, e14 (2012).

    7. Karch, C.M., Jeng, A.T., and Goate, A.M. Calcium phosphatase calcineurin influences tau metabolism. Neurobiol. Aging 34(2), 374-386 (2013).