MMP-1 (human, recombinant)

Matrix metalloproteinase-1 (MMP-1) is an endopeptidase that has a major role in tissue remodeling.^1,2 It is composed of an N-terminal extracellular signal peptide, a prodomain containing a cysteine switch that interacts with the catalytic domain to regulate the proteolytic activity of MMP-1, a catalytic domain, and a C-terminal domain containing four hemopexin-like repeats which confer specificity for collagen.¹ MMP-1 is ubiquitously expressed at low levels under normal physiological conditions and localizes to the extracellular space.² It is primarily involved in the degradation of the extracellular matrix with type I collagen being its main substrate, but also degrades types II, III, V, IX, and X fibrillar collagens. MMP-1 also cleaves cell surface molecules such as insulin-like growth factor 1 (IGF-1) binding protein 3 (IGFBP3) and TNF-α.¹ Knockdown of MMP1 inhibits the proliferation, migration, and invasion of colorectal cancer cells.³ MMP1 transgenic mice exhibit increased alveolar damage following exposure to M. tuberculosis.⁴ Polymorphisms of MMP1 are associated with several diseases, including various cancers, periodontitis, and coronary artery disease.² Cayman’s MMP-1 (human, recombinant) protein provided as the latent zymogen form. After activation with p-aminophenylmercuric acetate (APMA), it can be used for enzyme activity applications. This protein consists of 461 amino acids and has a predicted N-terminus of Phe20 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 50-55 kDa.

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1. Pardo, A., and Selman, M. MMP-1: The elder of the family. Int. J. Biochem. Cell Biol. 37(2), 283-288 (2005).

2. Arakaki, P.A., Marques, M.R., and Santos, M.C.L.G. MMP-1 polymorphism and its relationship to pathological processes. J. Biosci. 34(2), 313-320 (2009).

3. Wang, K., Zheng, J., Yu, J., et al. Knockdown of MMP‑1 inhibits the progression of colorectal cancer by suppressing the PI3K/Akt/c‑myc signaling pathway and EMT. Oncol. Rep. 43(4), 1103-1112 (2020).

4. Elkington, P., Shiomi, T., Breen, R., et al. MMP-1 drives immunopathology in human tuberculosis and transgenic mice. J. Clin. Invest. 121(5), 1827-1833 (2011).