p70S6K (N-Term) Rabbit Monoclonal Antibody (Clone RM438)

p70 Ribosomal S6 kinase (p70S6K) is serine/threonine kinase of the S6K family.¹ It is composed of an N-terminal domain, a kinase domain, and a C-terminal domain and has several phosphorylation sites. There are three isoforms of p70S6K with p70S6K1 and p70S6K2 generated by alternative translation initiation sites and p70S6K3 generated via alternative splicing. p70S6K localizes to the cytoplasm and is activated in response to insulin and growth factor signaling, as well as to amino acids and glucose metabolism.^2,1 It is activated in a process requiring phosphorylation of three or four serine residues in the C-terminal domain, depending on the isoform, which induces a conformational change that allows two threonine residues to be phosphorylated by mammalian target of rapamycin (mTOR) and 3-phosphoinositide-dependent protein kinase 1 (PDK1).^1,3 Active p70S6K phosphorylates the 40S ribosomal subunit ribosomal protein S6 (RSK), leading to protein synthesis and cell cycle progression.^2,1 p70S6K also targets proteins involved in other processes, including cell growth and motility, adipocyte differentiation, and synaptic plasticity.³ Expression of RPS6KB1, the gene encoding p70S6K1, is increased in visceral adipose tissue from obese patients.⁴ Cayman’s p70S6K (N-Term) Rabbit Monoclonal Antibody (RM438) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

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1. Tavares, M.R., Pavan, I.C.B., Amaral, C.L., et al. The S6K protein family in health and disease. Life Sci. 131, 1-10 (2015).

2. Long, X., Muller, F., and Avrüch, J. TOR action in mammalian cells and in Caenorhabditis elegans. TOR Target of Rapamycin 279, 115-138 (2004).

3. Magnuson, B., Ekim, B., and Fingar, D.C. Regulation and function of ribosomal protein S6 kinase (S6K) within mTOR signalling networks. Biochem. J. 441(1), 1-21 (2012).

4. Catalán, V., Gómez-Ambrosi, J., Rodríguez, A., et al. Expression of S6K1 in human visceral adipose tissue is upregulated in obesity and related to insulin resistance and inflammation. Acta Diabetol. 52(2), 257-266 (2015).