A fluorogenic substrate for CYP1A2 and CYP3A
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7-Benzyloxy-4-(trifluoromethyl)coumarin

Item No. 35721

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-(phenylmethoxy)-4-(trifluoromethyl)-2H-1-benzopyran-2-one
CAS Number
220001-53-6
Synonyms
  • BFC
Molecular Formula
C17H11F3O3
Formula Weight
320.3
Purity
≥95%
Emission
510 nm
Excitation
410 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 3 mg/mlDMSO: 1 mg/ml
SMILES
O=C1OC2=C(C(C(F)(F)F)=C1)C=CC(OCC3=CC=CC=C3)=C2
InChi Code
InChI=1S/C17H11F3O3/c18-17(19,20)14-9-16(21)23-15-8-12(6-7-13(14)15)22-10-11-4-2-1-3-5-11/h1-9H,10H2
InChi Key
WVKLERKKJXUPIK-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    10 mg

    $82

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    25 mg

    $182

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    50 mg

    $283

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    100 mg

    $482

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    Product Description

    7-Benzyloxy-4-(trifluoromethyl)coumarin (BFC) is a fluorogenic substrate for the cytochrome P450 (CYP) isoforms CYP1A2 and CYP3A.1,2 Upon enzymatic cleavage by CYP1A2 or CYP3A, 7-hydroxy-4-(trifluoromethyl)coumarin (HFC) is released and its fluorescence can be used to quantify CYP1A2 and CYP3A activity. HFC displays excitation/emission maxima of 410/510 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Renwick, A.B., Surry, D., Price, R.J., et al. Metabolism of 7-benzyloxy-4-trifluoromethyl-coumarin by human hepatic cytochrome P450 isoforms. Xenobiotica 30(10), 955-969 (2000).

    2. Ducharme, J., Sevrioukova, I.F., Thibodeaux, C.J., et al. Structural dynamics of cytochrome P450 3A4 in the presence of substrates and cytochrome P450 reductase. Biochemistry 60(28), 2259-2271 (2021).