For immunochemical detection of EGFR (phospho-Tyr1068)
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EGFR (Phospho-Tyr1068) Rabbit Monoclonal Antibody (Clone RM443)

Item No. 35901

Product Insert (PDF)
Technical Information
Synonyms
  • Epidermal Growth Factor Receptor
  • ErbB-1
  • Erb-B2 Receptor Tyrosine Kinase 1
  • HER1
Immunogen
A phospho-peptide corresponding to human phospho-EGFR
Clone Designation
RM443
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
WB
Cross Reactivity
(+) EGFR (Phospho-Tyr1068)(-) EGFR without phosphorylation at Tyr1068
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

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    Product Description

    Epidermal growth factor receptor (EGFR), also known as HER1 and ErbB-1, is a cell surface receptor and member of the EGF family of receptor tyrosine kinases with roles in cell proliferation, differentiation, and survival.1,2 It is a transmembrane receptor composed of an intracellular tyrosine kinase domain, a transmembrane lipophilic segment, and an extracellular domain that is expressed in epithelial, mesenchymal, and neuronal tissues.1,2,3 Under unstimulated conditions, EGFR is an auto-inhibited monomer in the plasma membrane.1 Upon canonical ligand binding, EGFR undergoes homodimerization or heterodimerization with HER2, HER3, or HER4, which induces a conformational change in the cytoplasmic domain that facilitates autophosphorylation and intracellular signaling. EGFR contains five C-terminal autophosphorylation sites, namely tyrosine 1068 (Tyr1068), Tyr1148, Tyr1173, Tyr1086, and Tyr992.4 EGFR (phospho-Tyr1068) is associated with prolonged progression-free survival in patients with non-small cell lung cancer (NSCLC). Cayman’s EGFR (Phospho-Tyr1068) Rabbit Monoclonal Antibody can be used for Western blot (WB).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sigismund, S., Avanzato, D., and Lanzetti, L. Emerging functions of the EGFR in cancer. Mol. Oncol. 12(1), 3-20 (2018).

    2. Herbst, R.S. Review of epidermal growth factor receptor biology. Int. J. Radiat. Oncol. Biol. Phys. 59(2 Suppl), 21-26 (2004).

    3. Yano, S., Kondo, K., Yamaguchi, M., et al. Distribution and function of EGFR in human tissue and the effect of EGFR tyrosine kinase inhibition. Anticancer Res. 23(5A), 3639-3650 (2003).

    4. Wang, F., Wang, S., Wang, Z., et al. Phosphorylated EGFR expression may predict outcome of EGFR-TKIs therapy for the advanced NSCLC patients with wild-type EGFR. J. Exp. Clin. Cancer Res. 31(1), 65 (2012).