For immunochemical detection of Aurora A (Phospho-Thr288)/Aurora B (Phospho-Thr232)/Aurora C (Phospho-Thr198)
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Aurora A (Phospho-Thr288)/Aurora B (Phospho-Thr232)/Aurora C (Phospho-Thr198) Rabbit Monoclonal Antibody (Clone RM454)

Item No. 35904

Product Insert (PDF)
Technical Information
Immunogen
A phospho-peptide corresponding to Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198)
Clone Designation
RM454
Formulation (Request formulation change)
100 µl protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198)(-) Aurora A/Aurora B/Aurora C without phosphorylation at Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198)
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

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    Cayman Chemical
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    Product Description

    The Aurora kinases, Aurora A, -B, and -C, are a family of serine/threonine kinases encoded by distinct genes, AURKA, AURKB, and AURKC, respectively, that have roles in mitosis and meiosis.1,2 They are composed of an ATP-binding N-terminal domain, a highly conservated catalytic domain, and a short C-terminal domain that contains an activation loop and mediates cofactor binding.1 Each Aurora kinase contains a key threonine residue in its C-terminal activation loop, threonine 288 (Thr288), Thr232, and Thr198 in Aurora A, -B, and -C, respectively, that is autophosphorylated upon cofactor binding, inducing a conformational change that mediates kinase activation.1,2 Aurora A and Aurora B have roles in mitosis, whereas Aurora C regulates meiosis.2 Aurora A and Aurora B are constitutively expressed in mitotic cells, where Aurora A localizes to the spindle poles and Aurora B localizes to the central spindle.2 Aurora A has roles in mitotic entry, centrosome maturation and separation, and spindle assembly, whereas Aurora B regulates chromosome condensation and alignment, spindle assembly, and cytokinesis. Aurora C is expressed in germ cells during meiosis, localizes to the chromosome midbody, and is required for spermatogenesis.2,3 Aurora A, -B, and -C are overexpressed in a variety of cancers, including breast, colorectal, ovarian, and cervical cancers, and the pan-Aurora kinase inhibitor AMG 900 (Item No. 19176) inhibits tumor growth in an HCT116 mouse xenograft model.3,4 Cayman's Aurora A (Phospho-Thr288)/Aurora B (Phospho-Thr232)/Aurora C (Phospho-Thr198) Rabbit Monoclonal Antibody (Clone RM454) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Willems, E., Dedobbeleer, M., Digregorio, M., et al. The functional diversity of Aurora kinases: A comprehensive review. Cell Div. 13, 7 (2018).

    2. Goldenson, B., and Crispino, J.D. The aurora kinases in cell cycle and leukemia. Oncogene 34(5), 537-545 (2015).

    3. Tang, A., Gao, K., Chu, L., et al. Aurora kinases: Novel therapy targets in cancers. Oncotarget 8(14), 23937-23954 (2017).

    4. Payton, M., Bush, T.L., Chung, G., et al. Preclinical evaluation of AMG 900, a novel potent and highly selective pan-aurora kinase inhibitor with activity in taxane-resistant tumor cell lines. Cancer Res. 70(23), 9846-9854 (2010).