COX-2 (ovine) Electrophoresis Standard

Cyclooxygenase 2 (COX-2) is a bifunctional enzyme that exhibits both COX and peroxidase activities and catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins.^1,2 The COX component converts arachidonic acid to the hydroperoxy endoperoxide prostaglandin G₂ (PGG₂; Item No. 17010), and the peroxidase component reduces the endoperoxide to the corresponding alcohol PGH₂ (Item No. 17020). COX2 expression is induced by a variety of stimuli, including phorbol esters, LPS, and cytokines and is responsible for the biosynthesis of PGs under acute inflammatory conditions.^3,4 Thus, COX-2 has been the focus of attention for nonsteroidal anti-inflammatory drug (NSAID) development. This product is intended to be used as a standard for Western blot (WB) and electrophoresis. This enzyme has been carefully purified to exclude all unrelated proteins and may not be catalytically active.

WARNING This product is not for human or veterinary use.

1. Nugteren, D.H., and Hazelhof, E. Isolation and properties of intermediates in prostaglandin biosynthesis. Biochim. Biophys. Acta 326(3), 448-461 (1973).

2. Hamberg, M., and Samuelsson, B. Detection and isolation of an endoperoxide intermediate in prostaglandin biosynthesis. Proc. Natl. Acad. Sci. USA 70(3), 899-903 (1973).

3. Kang, Y.-J., Mbonye, U.R., DeLong, C.J., et al. Regulation of intracellular cyclooxygenase levels by gene transcription and protein degradation. Prog. Lipid Res. 46(2), 108-125 (2007).

4. Blobaum, A.L., and Marnett, L.J. Structural and functional basis of cyclooxygenase inhibition. J. Med. Chem. 50(7), 1425-1441 (2007).

Kim, Y.P., Yamada, M., Lim, S.S., et al. Inhibition by tectorigenin and tectoridin of prostaglandin E₂ production and cyclooxygenase-2 induction in rat peritoneal macrophages. Biochim. Biophys. Acta 1438(3), 399-407 (1999).