A fluorometric reagent for selenium and nitric oxide
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2,3-Diaminonaphthalene

Item No. 36265

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2,3-naphthalenediamine
CAS Number
771-97-1
Synonyms
  • DAN
  • NSC 62692
Molecular Formula
C10H10N2
Formula Weight
158.2
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 10 mg/mlDMSO: 14 mg/ml
λmax
214, 247 nm
SMILES
NC1=CC2=C(C=C1N)C=CC=C2
InChi Code
InChI=1S/C10H10N2/c11-9-5-7-3-1-2-4-8(7)6-10(9)12/h1-6H,11-12H2
InChi Key
XTBLDMQMUSHDEN-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    Cayman Chemical
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    Product Description

    2,3-Diaminonaphthalene (DAN) is a fluorometric reagent for selenium and nitric oxide (NO).1,2,3 Upon binding to selenium, piazselenol is formed, which displays excitation/emission maxima of 365 and 525 nm, respectively.2 Upon binding to nitrite, the end product of the reaction between NO and oxygen in aqueous media, 1-(H)-naphthotriazole is formed, which displays excitation/emission maxima of 360 and 440 nm, respectively.3 DAN has been used in the detection of selenium in river water samples.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Suzuki, Y., Hashigaya, N., and Kawakubo, S. Development of a simple and low-cost device for fluorometric determination of selenium in water samples. Anal. Sci. 26(6), 719-722 (2010).

    2. Bayfield, R.F., and Romalis, L.F. pH Control in the fluorometric assay for selenium with 2,3-diaminonaphthalene. Anal. Biochem. 144(2), 569-576 (1985).

    3. Kleinhenz, D.J., Fan, X., Rubin, J., et al. Detection of endothelial nitric oxide release with the 2,3-diaminonapthalene assay. Free Radic. Biol. Med. 34(7), 856-861 (2003).