For immunochemical detection of TfR1
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Transferrin Receptor Protein 1/CD71 Monoclonal Antibody (Clone 3F3-FMA)

Item No. 36655

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Cluster of Differentiation 71
  • p90
  • TfR1
  • TFRC
  • Transferrin Receptor Protein 1
Immunogen
Purified membrane fractions from OCI-LY7 cells
Clone Designation
3F3-FMA
MW
~85 kD
Formulation (Request formulation change)
100 µg of protein G-purified monoclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Mouse
Isotype
IgG1
Applications
IF
Cross Reactivity
(+) TfR1
Species Reactivity
(+) Human
UniProt Accession №
P02786
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $321

    Add
    Cayman Chemical
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    Product Description

    Transferrin receptor (TfR1), also known as CD71, is a homodimeric transmembrane receptor for transferrin (Item No. 32030) that facilitates iron delivery into cells and is encoded by TFRC in humans.1 It is composed of two TfR1 monomers, each containing a cytoplasmic tail, an internalization motif, a membrane-spanning portion, and a stalk region that covalently links the monomers. An extracellular ectodomain binds transferrin and drives TfR1 dimerization.1,2 TfR1 is ubiquitously expressed, except on mature red blood cells and certain terminally differentiated cells, with the highest expression on immature erythroid cells and in the placenta, and is involved in erythropoiesis, lymphocyte development, and hematopoietic expansion in the bone marrow.3,4 TfR1/transferrin-mediated iron transport contributes to the intracellular iron pool required for ferroptosis and the anti-TfR1 antibody (clone 3F3-FMA) has been used in combination with anti-malondialdehyde antibodies to identify ferroptotic cells in vitro and human cancer tissue in a mouse xenograft model.5 TFRC is overexpressed in various breast cancer tumors and gliomas and positively correlated with poor prognosis.6 Cayman’s Transferrin Receptor Protein 1/CD71 Monoclonal Antibody (Clone 3F3-FMA) can be used for immunofluorescence (IF) applications. The antibody recognizes TfR1 at approximately 85 kDa from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Luck, A.N., and Mason, A.B. Transferrin-mediated cellular iron delivery. Curr. Top. Membr. 69, 3-35 (2012).

    2. Gammella, E., Buratti, P., Cairo, G., et al. The transferrin receptor: The cellular iron gate. Metallomics 9(10), 1367-1375 (2017).

    3. Ponka, P., and Lok, C.N. The transferrin receptor: Role in health and disease. Int. J. Biochem. Cell Biol. 31(10), 1111-1137 (1999).

    4. Wang, S., He, X., Wu, Q., et al. Transferrin receptor 1-mediated iron uptake plays an essential role in hematopoiesis. Haematologica 105(8), 2071-2082 (2020).

    5. Feng, H., Schorpp, K., Jin, J., et al. Transferrin receptor is a specific ferroptosis marker. Cell Rep. 30(10), 3411-3423 (2020).

    6. Shen, Y., Li, X., Dong, D., et al. Transferrin receptor 1 in cancer: A new sight for cancer therapy. Am. J. Cancer Res. 8(6), 916-931 (2018).