A fluorescent peptide
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5-FAM-LPETGG (trifluoroacetate salt)

Item No. 36997

Technical Information
Formal Name
3',6'-dihydroxy-N-((S)-1-((S)-1-((4S,7S,8R)-8-hydroxy-4-(hydroxyamino)-7-(2-(2-(hydroxyamino)acetamido)acetamido)-5,6-dioxononanoyl)pyrrolidin-2-yl)-5-methyl-1,2-dioxohexan-3-yl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxamide, trifluoroacetate salt
Synonyms
  • 5-Carboxyfluorescein-LPETGG
Molecular Formula
C45H50N6O16 • XCF3COOH
Formula Weight
Purity
≥90%
Emission
518 nm
Excitation
492 nm
A solid
DMF: 10 mg/mlDMSO: 10 mg/mlEthanol: 10 mg/mlPBS (pH 7.2): 5 mg/ml
λmax
230, 452 nm
SMILES
OC(C(F)(F)F)=O.O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=C(C(N[C@H](C(N6CCC[C@H]6C(N[C@@H](CCC(O)=O)C(N[C@]([C@H](O)C)([H])C(NCC(NCC(O)=O)=O)=O)=O)=O)=O)CC(C)C)=O)C=C5
InChi Code
InChI=1S/C45H50N6O16.C2HF3O2/c1-21(2)15-31(49-39(60)23-6-9-27-26(16-23)44(65)67-45(27)28-10-7-24(53)17-33(28)66-34-18-25(54)8-11-29(34)45)43(64)51-14-4-5-32(51)41(62)48-30(12-13-36(56)57)40(61)50-38(22(3)52)42(63)47-19-35(55)46-20-37(58)59;3-2(4,5)1(6)7/h6-11,16-18,21-22,30-32,38,52-54H,4-5,12-15,19-20H2,1-3H3,(H,46,55)(H,47,63)(H,48,62)(H,49,60)(H,50,61)(H,56,57)(H,58,59);(H,6,7)/t22-,30+,31+,32+,38+;/m1./s1
InChi Key
FWXOBYHRXRJQCE-IBJQTDBTSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    Product Description

    5-FAM-LPETGG is a fluorescent peptide composed of the sortase recognition sequence LPETGG coupled to the fluorescent label 5-carboyxfluorescein (5-FAM; Item No. 19581), which displays excitation/emission maxima of 492/518 nm, respectively.1 It has been used for site-specific labeling of target proteins via sortase-catalyzed transpeptidation.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Antos, J.M., Chew, G.-L., Guimaraes, C.P., et alSite-specific N- and C-terminal labeling of a single polypeptide using sortases of different specificity. J. Am. Chem. Soc. 131(31), 10800-10801 (2009).