For immunochemical detection of glypican-3
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Glypican-3 Rabbit Monoclonal Antibody (PE) (Clone 024)

Item No. 37008

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • GPC3
  • MXR7
  • OCI-5
Immunogen
A synthetic peptide corresponding to the central region of human glypican-3
Clone Designation
024
Formulation (Request formulation change)
Protein A-affinity purified monoclonal antibody
Storage Buffer
PBS solution with 0.5% BSA and 0.03% ProClin™ 300
Host
Rabbit
Isotype
IgG
Applications
FC
Cross Reactivity
(+) Glypican-3
Species Reactivity
(+) Human
UniProt Accession №
P51654
Shipping & Storage Information
Storage
2°C to 8°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    25 test

    $110

    Add

    100 test

    $220

    Add
    Cayman Chemical
    Visit Our Cancer Resource Center
    Find Tools & Resources to Study the Hallmarks of Cancer
    • Cancer cell signaling & regulation
    • Cancer metabolism
    • Tumor microenvironment
    EXPLORE NOW
    Product Description

    Glypican-3 (GPC3) is a membrane-bound heparan sulfate proteoglycan.1 It is composed of a signal peptide for membrane translocation, a cysteine-rich domain that contains a proteolytic cleavage site for proprotein convertases, a stalk region that contains heparan sulfate attachment sites, and a signal sequence for glycosylphosphatidylinositol (GPI) attachment, which anchors it to the cell surface. GPC3 is ubiquitously expressed during embryonic development and is only expressed in select adult tissues, including gastric glands and kidney tubules.2,3 It is involved in canonical and non-canonical Wnt signaling and binds to various Wnt ligands and Frizzled receptors.1,4 GPC3-targeting antibodies induce antibody-dependent cellular cytotoxicity (ADCC) and reduce tumor growth in hepatocellular carcinoma (HCC) mouse xenograft models.5 Hepatic levels of GPC3 are increased in patients with HCC.6 Mutations in GPC3 are associated with Simpson-Golabi-Behmel syndrome (SGBS), an X-linked condition characterized by pre- and post-natal overgrowth.2 Cayman’s Glypican-3 Rabbit Monoclonal Antibody (PE) (Clone 024) is composed of a GPC3 monoclonal antibody conjugated to phycoerythrin (PE) and can be used for flow cytometry (FC).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. De Cat, B., Muyldermans, S.-Y., Coomans, C., et al. Processing by proprotein convertases is required for glypican-3 modulation of cell survival, Wnt signaling, and gastrulation movements. J. Cell. Biol. 163(3), 625-635 (2003).

    2. Pilia, G., Hughes-Benzie, R.M., MacKenzie, A., et al. Mutations in GPC3, a glypican gene, cause the Simpson-Golabi-Behmel overgrowth syndrome. Nat. Genet. 12(3), 241-247 (1996).

    3. Baumhoer, D., Tornillo, L., Stadlmann, S., et al. Glypican 3 expression in human nonneoplastic, preneoplastic, and neoplastic tissues: A tissue microarray analysis of 4,387 tissue samples. Am. J. Clin. Pathol. 129(6), 899-906 (2008).

    4. Capurro, M., Martin, T., Shi, W., et al. Glypican-3 binds to frizzled and plays a direct role in the stimulation of canonical Wnt signaling. J. Cell. Sci. 127 (Pt. 7), 1565-1575 (2014).

    5. Ishiguro, T., Sugimoto, M., Kinoshita, Y., et al. Anti-glypican 3 antibody as a potential antitumor agent for human liver cancer. Cancer Res. 68(23), 9832-9838 (2008).

    6. Capurro, M., Wanless, I.R., Sherman, M., et al. Glypican-3: A novel serum and histochemical marker for hepatocellular carcinoma. Gastroenterology 125(1), 89-97 (2003).