For immunochemical detection of glypican-3
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Glypican-3 Rabbit Monoclonal Antibody (APC) (Clone 024)

Item No. 39540

Product Insert (PDF)
Technical Information
Synonyms
  • GPC3
  • MXR7
  • OCI-5
Immunogen
A synthetic peptide corresponding to the central region of human glypican-3
Clone Designation
024
Formulation (Request formulation change)
Protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 0.5% BSA and 0.03% ProClin™ 300
Host
Rabbit
Isotype
IgG
Applications
FCM
Cross Reactivity
(+) Glypican-3
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
2°C to 8°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    25 test

    $173

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    100 test

    $380

    Add
    Cayman Chemical
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    Product Description

    Glypican-3 (GPC3) is a membrane-bound heparan sulfate proteoglycan.1 It is composed of a signal peptide for membrane translocation, a cysteine-rich domain that contains a proteolytic cleavage site for proprotein convertases, a stalk region that contains heparan sulfate attachment sites, and a signal sequence for glycosylphosphatidylinositol (GPI) attachment, which anchors it to the cell surface. GPC3 is ubiquitously expressed during embryonic development and is only expressed in select adult tissues, including gastric glands and kidney tubules.2,3 It is involved in canonical and non-canonical Wnt signaling and binds to various Wnt ligands and Frizzled receptors.1,4 GPC3-targeting antibodies induce antibody-dependent cellular cytotoxicity (ADCC) and reduce tumor growth in hepatocellular carcinoma (HCC) mouse xenograft models.5 Hepatic levels of GPC3 are increased in patients with HCC.6 Mutations in GPC3 are associated with Simpson-Golabi-Behmel syndrome (SGBS), an X-linked condition characterized by pre- and post-natal overgrowth.2 Cayman’s Glypican-3 Rabbit Monoclonal Antibody (APC) (Clone 024) is composed of a GPC3 monoclonal antibody conjugated to allophycocyanin (APC) and can be used for flow cytometry (FC).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. De Cat, B., Muyldermans, S.-Y., Coomans, C., et al. Processing by proprotein convertases is required for glypican-3 modulation of cell survival, Wnt signaling, and gastrulation movements. J. Cell. Biol. 163(3), 625-635 (2003).

    2. Pilia, G., Hughes-Benzie, R.M., MacKenzie, A., et al. Mutations in GPC3, a glypican gene, cause the Simpson-Golabi-Behmel overgrowth syndrome. Nat. Genet. 12(3), 241-247 (1996).

    3. Baumhoer, D., Tornillo, L., Stadlmann, S., et al. Glypican 3 expression in human nonneoplastic, preneoplastic, and neoplastic tissues: A tissue microarray analysis of 4,387 tissue samples. Am. J. Clin. Pathol. 129(6), 899-906 (2008).

    4. Capurro, M., Martin, T., Shi, W., et al. Glypican-3 binds to frizzled and plays a direct role in the stimulation of canonical Wnt signaling. J. Cell. Sci. 127 (Pt. 7), 1565-1575 (2014).

    5. Ishiguro, T., Sugimoto, M., Kinoshita, Y., et al. Anti-glypican 3 antibody as a potential antitumor agent for human liver cancer. Cancer Res. 68(23), 9832-9838 (2008).

    6. Capurro, M., Wanless, I.R., Sherman, M., et al. Glypican-3: A novel serum and histochemical marker for hepatocellular carcinoma. Gastroenterology 125(1), 89-97 (2003).