A fluorogenic substrate for the 20S proteasome and calpains
Related Products
Alternative(s)
10008119Suc-Leu-Leu-Val-Tyr-AMC
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Suc-Leu-Leu-Val-Tyr-AMC (trifluoroacetate salt)

Item No. 37043

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-(3-carboxy-1-oxopropyl)-L-leucyl-L-leucyl-L-valyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-tyrosinamide, trifluoroacetate salt
Synonyms
  • Suc-LLVY-AMC
Molecular Formula
C40H53N5O10 • XCF3COOH
Formula Weight
763.9
Purity
≥98%
Emission
440-460 nm
Excitation
340-360 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 2 mg/mlDMSO: 2 mg/ml
λmax
324 nm
SMILES
CC(C1=CC=C(C=C1O2)NC([C@H](CC3=CC=C(C=C3)O)NC([C@@H](NC([C@@H](NC([C@@H](NC(CCC(O)=O)=O)CC(C)C)=O)CC(C)C)=O)C(C)C)=O)=O)=CC2=O.FC(F)(F)C(O)=O
InChi Code
InChI=1S/C40H53N5O10.C2HF3O2/c1-21(2)16-29(42-33(47)14-15-34(48)49)38(52)43-30(17-22(3)4)39(53)45-36(23(5)6)40(54)44-31(19-25-8-11-27(46)12-9-25)37(51)41-26-10-13-28-24(7)18-35(50)55-32(28)20-26;3-2(4,5)1(6)7/h8-13,18,20-23,29-31,36,46H,14-17,19H2,1-7H3,(H,41,51)(H,42,47)(H,43,52)(H,44,54)(H,45,53)(H,48,49);(H,6,7)/t29-,30-,31-,36-;/m0./s1
InChi Key
DIIZONRJGINNAC-JJCSBACLSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    5 mg

    $94

    Add

    10 mg

    $179

    Add

    25 mg

    $422

    Add

    50 mg

    $748

    Add
    Product Description

    Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate for the 20S proteasome, other chymotrypsin-like proteases, and calpains.1,2 Upon enzymatic cleavage, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify protease activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Geier, E., Pfeifer, G., Wilm, M., et al. A giant protease with potential to substitute for some functions of the proteasome. Science 283(5404), 978-981 (1999).

    2. Sasaki, T., Kikuchi, T., Yumoto, N., et al. Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates. The Journal of Biological Chemisty 259(20), 12489-12494 (1984).