Active • Host: Insect cells • AA: 1-556 (full length) • Tag: N-terminal His • MW: 66.7 kDa
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PDK1 (human, recombinant)

Item No. 37315

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • 3-Phosphoinositide-dependent Protein Kinase 1
Purity
≥85% estimated by SDS-PAGE
Source
Active recombinant human N-terminal His-tagged PDK1 expressed in insect cells
Amino Acids
1-556 (full length)
MW
66.7 kDa
Formulation (Request formulation change)
50 mM Tris-HCl, pH 7.4, with 150 mM sodium chloride and 10% glycerol
UniProt Accession №
O15530
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    10 µg

    $396

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    Product Description

    3-Phosphoinositide-dependent protein kinase 1 (PDK1) is a serine/threonine kinase with roles in cell survival, differentiation, and proliferation, metabolic regulation, and immune development.1,2,3 It is composed of an N-terminal bilobular kinase domain, with the small lobe housing a PIF-pocket/αC-helix region and the large lobe containing an activation loop, and a C-terminal PH domain.1 PDK1 is activated by autophosphorylation of serine 241 in the activation loop, a residue that is poorly accessible to phosphatases, and is considered constitutively active.1 It phosphorylates and activates various members of the AGC protein kinase family, including Akt, p70 ribosomal S6 kinase (p70S6K), serum/glucocorticoid regulated kinase (SGK), and PKC to regulate the PI3K/Akt, Ras/MAPK, and Myc signaling pathways.1,2 Gene amplification of PDPK1, the gene encoding PDK1, is associated with poor prognosis in patients with breast cancer and metastasis in patients with prostate cancer.1 Knockdown of Pdpk1 promotes axon regeneration in a mouse model of sciatic nerve injury and inhibits T follicular helper (Tfh) cell differentiation and germinal center responses in a mouse model of acute lymphocytic choriomeningitis virus (LCMV) infection.2,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Gagliardi, P.A., Puliafito, A., and Primo, L. PDK1: At the crossroad of cancer signaling pathways. Semin. Cancer Biol. 48, 27-35 (2018).

    2. Kim, H., Lee, J., and Cho, Y. PDK1 is a negative regulator of axon regeneration. Mol. Brain 14(1), 31 (2021).

    3. Sun, Z., Yao, Y., You, M., et al. The kinase PDK1 is critical for promoting T follicular helper cell differentiation. Elife 10, e61406 (2021).