Active • Host: HEK293 cells • AA: 32-314 • Tag: C-terminal His • MW: 34 kDa
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sFRP1 (human, recombinant)

Item No. 38061

Product Insert (PDF)
Technical Information
Synonyms
  • SARP-2
  • Secreted Apoptosis-related Protein 2
  • Secreted Frizzled-related Protein 1
Purity
≥97% as determined by SDS-PAGE
Endotoxin Testing
<1.0 EU/µg, determined by the LAL endotoxin assay
Source
Active recombinant human C-terminal His-tagged sFRP1 expressed in HEK293 cells
Amino Acids
32-314
MW
34 kDa
Formulation (Request formulation change)
Lyophilized from sterile PBS, pH 7.4
Specific Activity
5-30 μg/ml (ED50)
UniProt Accession №
Q8N474
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    50 µg

    $273

    Add
    Cayman Chemical
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    Product Description

    Secreted frizzled-related protein 1 (sFRP1) is a Wnt inhibitor and a member of the sFRP family.1,2 It is composed of a signal peptide, a cysteine-rich domain, and a netrin-like domain.1 sFRP1 is ubiquitously expressed, with the highest levels in the heart, ovary, testis, and small intestine.3 It inhibits Wnt signaling by sequestering Wnt directly via the cysteine-rich or netrin-like domains or competing with Wnt for binding to the cysteine-rich domain of Frizzled (Fz) receptors.1 sFRP1 is involved in apoptosis, adipocyte differentiation, angiogenesis, osteoblast proliferation and differentiation, development, and hematopoietic stem cell maintenance.1,2 Myocardial overexpression of Sfrp1 protects against decreases in cardiac function and increases in myocardium apoptosis in a mouse model of heart failure induced by transverse aortic constriction.4 Tumor protein levels of sFRP1 are decreased in early-stage breast cancer, which is associated with poor prognosis.5 Cayman’s sFRP1 (human, recombinant) protein can be used for cell-based assays. This protein consists of 294 amino acids, has a calculated molecular weight of 34 kDa, and a predicted N-terminus of Ser32 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 38 kDa due to glycosylation.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Guan, H., Zhang, J., Luan, J., et al. Secreted frizzled related proteins in cardiovascular and metabolic diseases. Front. Endocrinol. 12, 712217 (2021).

    2. Bodine, P.V.N., and Komm, B.S. Wnt signaling and osteoblastogenesis. Rev. Endocr. Metab. Disord. 7(1-2), 33-39 (2006).

    3. Melkonyan, H.S., Chang, W.C., Shapiro, J.P., et al. SARPs: A family of secreted apoptosis-related proteins. Proc. Natl. Acad. Sci. USA 94(25), 13636-13641 (1997).

    4. Pan, S., Zhao, X., Wang, X., et al. Sfrp1 attenuates TAC-induced cardiac dysfunction by inhibiting Wnt signaling pathway- mediated myocardial apoptosis in mice. Lipids Health Dis. 17(1), 202 (2018).

    5. Klopocki, E., Kristiansen, G., Wild, P.J., et al. Loss of SFRP1 is associated with breast cancer progression and poor prognosis in early stage tumors. Int. J. Oncol. 25(3), 641-649 (2004).