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BSA-Stearate Saturated Fatty Acid Complex (1 mM) is composed of stearic acid (Item No. 10011298) and fatty acid-free bovine serum albumin (BSA) at an approximately 6:1 molar ratio of stearate:BSA. It was prepared under sterile conditions, then filtered and aliquoted into sterile vials. Cayman's BSA-Stearate Saturated Fatty Acid Complex (1 mM) can be used for efficient fatty acid delivery to cells in culture for the purpose of monitoring lipid metabolism and inflammatory signaling pathways.1 BSA-stearate complexes can also be used in colony formation, migration, and senescence assays to study the role of stearic acid in cancer in vitro.2 Cayman's BSA-Stearate Saturated Fatty Acid Complex (1 mM) is suitable for use in short- and long-term cell culture applications (25+ hours). For best results, it is recommended that this product be used in conjunction with Cayman's BSA Control for BSA-Fatty Acid Complexes (1 mM) (Item No. 34932), prepared with fatty acid-free BSA.
WARNING This product is not for human or veterinary use.
1. Cell culture models of fatty acid overload: Problems and solutions. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1863(2), 143-151 (2018).
2. Metabolic flux analysis reveals the roles of stearate and oleate on CPT1C-