A BSA complex with stearic acid
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BSA-Stearate Saturated Fatty Acid Complex (0.34 mM)

Item No. 43451

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Bovine Serum Albumin-SA
  • Bovine Serum Albumin-Stearate
  • BSA-SA
  • BSA-Octadecanoic Acid
Formulation (Request formulation change)
0.34 mM Stearate:0.17 mM BSA in 150 mM sodium chloride
Origin
Animal/Bovine
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    5 ml

    $197

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    Product Description

    BSA-Stearate Saturated Fatty Acid Complex (0.34 mM) is composed of stearic acid (Item No. 10011298) and fatty acid-free bovine serum albumin (BSA) at an approximately 2:1 molar ratio of stearate:BSA. It can be used for efficient fatty acid delivery to cells in culture for the purpose of monitoring lipid metabolism and inflammatory signaling pathways.1 It can also be used in colony formation, migration, and senescence assays to study the role of stearic acid in cancer in vitro.2 Cayman's BSA-Stearate Saturated Fatty Acid Complex (0.34 mM) is suitable for use in short-term cell culture applications (acute treatment to 18 hours); however, for long-term applications (25+ hours) the product should be filter-sterilized using a 0.2 µm filter and sterile receptacle, which will not affect its performance. When used at the same lipid concentration as BSA-Stearate Saturated Fatty Acid Complex (1 mM) (Item No. 38107), which has a 6:1 molar ratio of stearate:BSA, this product provides a lower free stearate concentration, which may reduce the likelihood of acute lipotoxicity.3 For best results, it is recommended that this product be used in conjunction with Cayman's BSA Control for BSA-Fatty Acid Complexes (1 mM) (Item No. 34932), prepared with fatty acid-free BSA.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Alsabeeh, N., Chausse, B., Kakimoto, P.A., et al. Cell culture models of fatty acid overload: Problems and solutions. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1863(2), 143-151 (2018).

    2. Chen, P., Tian, J., Zhou, Y., et al. Metabolic flux analysis reveals the roles of stearate and oleate on CPT1C-mediated tumor cell senescence. Int. J. Biol. Sci. 19(7), 2067-2080 (2023).

    3. Richieri, G.V., Anel, A., and Kleinfeld, A.M. Interactions of long-chain fatty acids and albumin: Determination of free fatty acid levels using the fluorescent probe ADIFAB. Biochemistry 32(29), 7574-7580 (1993).