PAMAM Dendrimer G5.0 Amine (water solution)

PAMAM dendrimer G5.0 amine (PAMAM G5.0) is a polyamidoamine (PAMAM) dendrimer with amine termini.¹ It is approximately 54 Å in diameter and has 128 surface groups. PAMAM G5.0 (10 µg/ml) reduces viability, induces cell cycle arrest at the sub-G₁/M phase, and increases lactate dehydrogenase (LDH) release from RAW 264.7 macrophages.² It also induces DNA fragmentation in the same cells when used at a concentration of 15 µg/ml. PAMAM G5.0 in complex with siRNA targeting Pou5f1 mRNA, the gene encoding Oct4, reduces Oct4 expression in mouse embryonic stem cells.³ Intravenous administration of PAMAM G5.0 in complex with reporter gene DNA increases reporter gene expression in the lung, spleen, and liver, but not the heart, in mice.⁴ Conjugates of PAMAM G5.0 with the contrast agent gadolinium increases small vessel contrast quality compared to conjugates of gadolinium with the cell-impermeable metal chelating agent diethylenetriaminepentaacetic acid (DTPA; Item No. 33307).⁵

WARNING This product is not for human or veterinary use.

1. Heiden, T.C., Dengler, E., Kao, W.J., et al. Developmental toxicity of low generation PAMAM dendrimers in zebrafish. Toxicol. Appl. Pharmacol. 225(1), 70-79 (2007).

2. Keo, J.-H.S., Jan, M.-S., and Chiu, H.W. Mechanism of cell death induced by cationic dendrimers in RAW 264.7 murine macrophage-like cells. J. Pharm. Pharmacol. 57(4), 489-495 (2004).

3. Ziraksaz, Z., Nomani, A., Soleimani, M., et al. Evaluation of cationic dendrimer and lipid as transfection reagents of short RNAs for stem cell modification. Int. J. Pharm. 448(1), 231-238 (2013).

4. Navarro, G., and Tros de Ilarduya, C. Activated and non-activated PAMAM dendrimers for gene delivery in vitro and in vivo. Nanomedicine 5(3), 287-297 (2009).

5. Sato, N., Kobayashi, H., Hiraga, A., et al. Pharmacokinetics and enhancement patterns of macromolecular MR contrast agents with various sizes of polyamidoamine dendrimer cores. Magn. Reson. Chem. 46(6), 1169-1173 (2001).