PAMAM Dendrimer G2.5 Carboxylate (sodium salt) (water solution)

PAMAM dendrimer G2.5 carboxylate is a polyamidoamine (PAMAM) dendrimer with carboxylate termini (PAMAM G2.5).¹ It is approximately 48.3 Å in diameter in water and has 24 surface groups.² PAMAM G2.5 binds to cytochrome c and decreases its hydrolysis by chymotrypsin by approximately ~30% in a cell-free assay when used at a concentration of 1 µM. When coupled to polyethylenimine (PEI), PAMAM G2.5 co-assembles with DNA into dendrosome nanoparticles (DNPs), which increase transfection efficiency and decrease DNA nuclease degradation in HeLa cells.³ When coupled to the adenosine A₁ receptor agonist adenosine amine congener (ADAC; Item No. 34394), PAMAM G2.5-ADAC inhibits forskolin-induced activation of adenylyl cyclase in CHO cells expressing the human adenosine A₃ receptor (EC₅₀ = 1.6 nM).⁴

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1. Chiba, F., Hu, T.C., Twyman, L.J., et al. Dendritic macromolecules as inhibitors to protein-protein binding. Macromol. Symp. 287(1), 37-41 (2010).

2. Caminati, G., Turro, N.J., and Tomalia, D.A. Photophysical investigation of starburst dendrimers and their interactions with anionic and cationic surfactants. J. Am. Chem. Soc. 112(23), 8515-8522 (1990).

3. Pan, S., Cao, D., Huang, H., et al. A serum-resistant low-generation polyamidoamine with PEI 423 outer layer for gene delivery vector. Macromol. Biosci. 13(4), 422-436 (2013).

4. Klutz, A.M., Gao, Z.-G., Lloyd, J., et al. Enhanced A₃ adenosine receptor selectivity of multivalent nucleoside-dendrimer conjugates. J. Nanobiotechnology 6, 12 (2008).