A chemiluminescent probe for the detection of superoxide
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L-012 (sodium salt)

Item No. 39908

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
8-amino-5-chloro-2,3-dihydro-7-phenyl-pyrido[3,4-d]pyridazine-1,4-dione, monosodium salt
CAS Number
143556-24-5
Molecular Formula
C13H8ClN4O2 • Na
Formula Weight
310.7
Purity
≥98%
Emission
470 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Acetonitrile: Slightly Soluble: 0.1-1 mg/ml
SMILES
[O-]C1=NNC(C2=C(C(C3=CC=CC=C3)=NC(Cl)=C12)N)=O.[Na+]
InChi Code
InChI=1S/C13H9ClN4O2.Na/c14-11-8-7(12(19)17-18-13(8)20)9(15)10(16-11)6-4-2-1-3-5-6;/h1-5H,15H2,(H,17,19)(H,18,20);/q;+1/p-1
InChi Key
IGEUYSJHQQCEFP-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $31

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    5 mg

    $131

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    10 mg

    $247

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    25 mg

    $462

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    Product Description

    L-012 is a chemiluminescent probe for the detection of superoxide and a derivative of luminol (Item No. 16803).1 It has commonly been used for the detection of NADPH oxidase-derived superoxide in vitro and for the detection of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in vivo.1,2,3 L-012 has a chemiluminescent emission maximum of 470 nm.3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Daiber, A., August, M., Baldus, S., et al. Measurement of NAD(P)H oxidase-derived superoxide with the luminol analogue L-012. Free Radic. Biol. Med. 36(1), 101-111 (2004).

    2. Kielland, A., Blom, T., Nandakumar, K.S., et al. In vivo imaging of reactive oxygen and nitrogen species in inflammation using the luminescent probe L-012. Free Radic. Biol. Med. 47(6), 760-766 (2009).

    3. Gao, L., Zhang, Y., Zhao, L., et al. An artificial metalloenzyme for catalytic cancer-specific DNA cleavage and operando imaging. Sci. Adv. 6(29), eabb1421 (2020).