Active • Host: Yeast • AA: 134-281 • Tag: N-terminal His • MW: 19.3 kDa
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Soluble Fas Ligand/CD95 Ligand (human, recombinant; aa 134-281)

Item No. 40212

Product Insert (PDF)
Technical Information
Synonyms
  • CD178
  • CD95L
  • FasL
  • FASLG
  • TNFL6
  • TNF Ligand Superfamily Member 6
  • TNFSF6
  • Tumor Necrosis Factor Ligand 6
Purity
≥90% estimated by SDS-PAGE
Source
Active recombinant human N-terminal His-tagged FasL expressed in yeast
Amino Acids
134-281
MW
19.3 KDa
Formulation (Request formulation change)
Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol, and 0.01% polysorbate 80
UniProt Accession №
P48023
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    50 µg

    $262

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    1 mg

    $2,618

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    Cayman Chemical
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    Product Description

    Fas ligand (FasL), also known as CD95L, is a type II transmembrane protein and member of the TNF superfamily.1 It is composed of a cytoplasmic domain, transmembrane domain, stalk region, TNF homology domain (THD), which is involved in homotrimerization, and Fas-binding domain. FasL is expressed in activated T cells, as well as the placenta, testis, lung, prostate, esophagus, uterus and spleen.2 Binding of FasL to its receptor Fas leads to formation of a death-inducing signaling complex (DISC) composed of CD95 oligomers, the Fas-associated death domain protein (FADD), and procaspase-8, procaspase-10, and cellular FLICE-like inhibitory protein (c-FLIP), which induces apoptosis.3 Soluble FasL (sFasL) is produced by proteolytic cleavage of FasL by various matrix metalloproteinases (MMPs) and either inhibits FasL-induced apoptosis or induces apoptosis in a context-dependent manner.1,4 sFasL induces migration of isolated human neutrophils and apoptosis in primary human distal lung epithelial cells.5,6 It decreases ankylosis in a rat model of collagen-induced arthritis.7 Serum levels of sFasL are increased in patients with insulin-dependent diabetes mellitus, systemic lupus erythematosus (SLE), rheumatoid arthritis, gastric cancer, or leukemia. Cayman’s Soluble Fas Ligand/CD95 Ligand (human, recombinant; aa 134-281) protein can be used for binding assays. This protein consists of 168 amino acids and has a calculated molecular weight of 19.3 kDa. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 26 kDa due to glycosylation.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Levoin, N., Jean, M., and Legembre, P. CD95 structure, aggregation and cell signaling. Front. Cell Dev. Biol. 8, 314 (2020).

    2. Xerri, L., Devilard, E., Hassoun, J., et al. Fas ligand is not only expressed in immune privileged human organs but is also coexpressed with Fas in various epithelial tissues. Mol. Pathol. 50(2), 87-91 (1997).

    3. Peter, M.E., Hadji, A., Murmann, A.E., et al. The role of CD95 and CD95 ligand in cancer. Cell Death Differ. 22(4), 549-559 (2015).

    4. Suda, T., Hashimoto, H., Tanaka, M., et al. Membrane Fas ligand kills human peripheral blood T lymphocytes, and soluble Fas ligand blocks the killing. J. Exp. Med. 186(12), 2045-2050 (1997).

    5. Ottonello, L., Tortolina, G., Amelotti, M., et al. Soluble Fas ligand is chemotactic for human neutrophilic polymorphonuclear leukocytes. J. Immunol. 162(6), 3601–3606 (1999).

    6. Matute-Bello, G., Liles, W.C., Steinberg, K.P., et al. Soluble Fas ligand induces epithelial cell apoptosis in humans with acute lung injury (ARDS). J. Immunol. 163(4), 2217-2225 (1999).

    7. Li, N.-L., Nie, H., Yu, Q.-W., et al. Role of soluble Fas ligand in autoimmune diseases. World J. Gastroenterol. 10(21), 3151-3156 (2004).