Host: Insect cells • AA: 1-298 (full length) • Tag: C-terminal His • MW: 35 kDa
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Cdk2 Isoform 1 (human, recombinant)

Item No. 40793

Product Insert (PDF)
Technical Information
Synonyms
  • Cell Division Protein Kinase 2
  • Cyclin-dependent Kinase 2
  • p33 Protein Kinase
Purity
≥85% estimated by SDS-PAGE, ≥95% determined by SEC-HPLC
Endotoxin Testing
<1.0 EU/µg, determined by the LAL endotoxin assay
Source
Recombinant human C-terminal His-tagged Cdk2 isoform 1 expressed in insect cells
Amino Acids
1-298 (full length)
MW
35 kDa
Formulation (Request formulation change)
Lyophilized from sterile 50 mM Tris, pH 8.0, with 100 mM sodium chloride and 10% glycerol
Host
Insect cells
UniProt Accession №
P24941
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µg

    $273

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    500 µg

    $1,774

    Add
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    Product Description

    Cyclin-dependent kinase 2 (Cdk2) is a serine/threonine kinase and member of the CDK family of kinases involved in cell cycle regulation.1,2 It is composed of an N-terminal domain, an activation segment, also known as the T loop, and a catalytic C-terminal domain.2 Alternative splicing of CDK2 mRNA produces a full-length isoform or a shorter isoform that lacks exon 5, which encodes for part of the Cdk2 activation segment and contains residues that participate in interactions with cyclins.3 It is ubiquitously expressed and localizes to the cytoplasm or nucleus depending on the cell cycle stage.4,5 Cdk2 associates with cyclin E to mediate the G1/S transition and with cyclin A during S phase progression.1,2 Knockout of Cdk2 induces gametogenesis defects in mice but does not affect mitotic cell division.6 Protein levels of Cdk2 are increased in patients with oral squamous cell carcinoma, and overexpression of Cdk2/cyclin A or Cdk2/cyclin E is associated with poor prognosis in patients with oral cancer.7 Cayman’s Cdk2 Isoform 1 (human, recombinant) protein consists of 308 amino acids and has a calculated molecular weight of 35 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Funk, J.O. Cell cycle checkpoint genes and cancer. Encyclopedia of life sciences 1-6 (2005).

    2. Li, Y., Zhang, J., Gao, W., et al. Insights on structural characteristics and ligand binding mechanisms of CDK2. Int. J. Mol. Sci. 16(5), 9314-9340 (2015).

    3. Ji, X., Humenik, J., Yang, D., et al. PolyC-binding proteins enhance expression of the CDK2 cell cycle regulatory protein via alternative splicing. Nucleic Acids Res. 46(4), 2030-2044 (2018).

    4. Sherr, C.J., and Roberts, J.M. Living with or without cyclins and cyclin-dependent kinase. Genes Dev. 18(22), 2699-2711 (2004).

    5. Bresnahan, W.A., Thompson, E.A., and Albrecht, T. Human cytomegalovirus infection results in altered Cdk2 subcellular localization. J. Gen. Virol. 78(Pt 8), 1993-1997 (1997).

    6. Ortega, S., Prieto, I., Odajima, J., et al. Cyclin-dependent kinase 2 is essential for meiosis but not for mitotic cell division in mice. Nat. Genet. 35(1), 25-31 (2003).

    7. Mihara, M., Shintani, S., Nakahara, Y., et al. Overexpressio0n of CDK2 Is a prognostic indicator of oral cancer progression. Jpn. J. Cancer Res. 92(3), 352-360 (2001).