A fluorescent DNA probe
Related Products
Alternative(s)
14285DAPI (hydrochloride)
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Product Categories

Product Type

Biochemicals
Labeling & Detection
Fluorescent Probes

Research Area

Application

Fluorescence

DAPI (hydrochloride) (solution)

Item No. 40796

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-[4-(aminoiminomethyl)phenyl]-1H-indole-6-carboximidamide, dihydrochloride
CAS Number
28718-90-3
Synonyms
  • 4',6-Diamidino-2-phenylindole
  • FxCycle Violet
Molecular Formula
C16H15N5 • 2HCl
Formula Weight
350.3
Purity
≥98%
Emission
453 nm
Excitation
347 nm
Formulation (Request formulation change)
A 5 mg/ml solution in DMSO
Solubility(Learn about Variance in Solubility)
DMSO: Sparingly soluble: 1-10 mg/ml
SMILES
NC(C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2)=N.Cl.Cl
InChi Code
InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChi Key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    1 mg

    $32

    Add
    Product Description

    DAPI is a fluorescent DNA probe.1,2 It forms a fluorescent complex by attaching in the minor groove of adenine and thymine-rich sequences of DNA.1 DAPI displays excitation/emission maxima of 347/453 nm, respectively. It has been commonly used as a cell viability dye and as a counterstain for DNA and chromosomes in fluorescent microscopy and flow cytometry applications.1,2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kapuscinski, J. DAPI: A DNA-specific fluorescent probe. Biotech. Histochem. 70(5), 220-233 (1995).

    2. Zink, D., Sadoni, N., and Stelzer, E. Visualizing chromatin and chromosomes in living cells. Methods 29(1), 42-50 (2003).