Glucocorticoid Receptor Bioassay

The principal application of this Glucocorticoid Receptor bioassay is to screen processed environmental samples, with the aim of quantifying the aggregate bioactivities, if any, of contaminating endocrine disrupting chemical (EDC) entities.

This assay utilizes proprietary mammalian cells expressing the native Glucocorticoid Receptor (GR; nr3c1), a ligand-dependent transcription factor. The cells also include the luciferase reporter gene functionally linked to a GR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in total GR activity.

The GR Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate steps such as spin-and-rinse steps, viability determinations, or cell titer adjustments prior to assay setup.

This GR Assay is an all-inclusive assay system. In addition to the Reporter Cells, included are two optimized media for use during cell culture and in diluting the user's test samples, the reference agonist Dexamethasone, Luciferase Detection Reagent, and a cell cultureready assay plate. [INDIGO Catalog No. EM00201]

WARNING This product is not for human or veterinary use.