Rabbit Peroxisome Proliferator-Activated Receptor Delta Reporter Assay System

This assay utilizes proprietary non-human cells engineered to provide constitutive, high level expression of Rabbit (Oryctolagus cuniculus) Peroxisome Proliferator-Activated Receptor Delta (nr1c2), a ligand-dependent transcription factor commonly referred to as PPARD or PPARδ.

INDIGO's Reporter Cells include the luciferase gene functionally linked to a responsive promoter. Thus, quantifying changes in luciferase expression in the treated cells provides a sensitive surrogate measure of the changes in PPARδ activity. The principal application of this assay is in the screening of test samples to quantify functional activity, either agonist or antagonist, that they may exert against rabbit PPARδ.

Rabbit PPARδ Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin and-rinse of cells, viability determinations, cell titer adjustments, or the pre-incubation of cells prior to assay setup.

INDIGO’s assay kits provide all-inclusive cell-based assay systems. In addition to Rabbit PPARδ Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user's test samples, the reference agonist GW0742, Luciferase Detection Reagent, and a cell culture-ready assay plate. [INDIGO Catalog Nos. RB00121 | RB00121-32]

WARNING This product is not for human or veterinary use.