A cell-permeable fluorogenic probe
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Alternative(s)
168025-CFSE
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6-CFSE

Item No. 41342

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3′,6′-dihydroxy-3-oxo-spiro[isobenzofuran-1(3H),9′-[9H]xanthene]-6-carboxylic acid, 2,5-dioxo-1-pyrrolidinyl ester
CAS Number
92557-81-8
Synonyms
  • 6-Carboxyfluorescein NHS ester
  • 6-Carboxyfluorescein N-hydroxysuccinimide ester
  • 6-Carboxyfluorescein N-Succinimidyl ester
  • 6-FAM SE
  • 6-FAM N-succinimidyl ester
Molecular Formula
C25H15NO9
Formula Weight
473.4
Purity
≥95%
Emission
518 nm
Excitation
492 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Acetonitrile: Slightly soluble: 0.1-1 mg/mlDMSO: Slightly soluble: 0.1-1 mg/ml
SMILES
OC1=CC=C2C3(C4=CC(C(ON5C(CCC5=O)=O)=O)=CC=C4C(O3)=O)C6=CC=C(O)C=C6OC2=C1
InChi Code
InChI=1S/C25H15NO9/c27-13-2-5-16-19(10-13)33-20-11-14(28)3-6-17(20)25(16)18-9-12(1-4-15(18)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2
InChi Key
VDABVNMGKGUPEY-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    5 mg

    $54

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    10 mg

    $97

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    50 mg

    $297

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    100 mg

    $486

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    Product Description

    6-CFSE is cell-permeable fluorogenic probe that is composed of the amine-reactive fluorescent probe 6-carboxyfluorescein (Item No. 21233) and a cleavable succinimidyl ester.1 It is cleaved by intracellular esterases to produce 6-carboxyfluorescein, which displays excitation/emission maxima of 492/518 nm, respectively. The racemic mixture of 6-CFSE and 5-CFSE (Item No. 16802) has been used to determine the intracellular pH of bacteria, quantify cell division in vitro and in vivo, and track lymphocyte migration in vivo.2,3,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Fischer, R., Mader, O., Jung, G., et al. Extending the applicability of carboxyfluorescein in solid-phase synthesis. Bioconjug. Chem. 14(3), 653-660 (2003).

    2. Breeuwer, P., Drocourt, J., Rombouts, F.M., et al. A novel method for continuous determination of the intracellular pH in bacteria with the internally conjugated fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester. Appl. Environ. Microbiol. 62(1), 178-183 (1996).

    3. Lyons, A.B. Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution. J. Immunol. Methods 243(1-2), 147-154 (2000).

    4. Parish, C.R., Glidden, M.H., Quah, B.J.C., et al. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Curr. Protoc. Immunol. Supp. 84, 4.9.1-4.9.13 (2009).