An inhibitor of USP28
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USP28-IN-4

Item No. 41604

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-chloro-N-[4-chloro-3-(2,3-dihydro-1H-isoindol-5-yl)phenyl]-4-(methylsulfonyl)-benzamide
CAS Number
2931509-15-6
Synonyms
  • Ubiquitin-specific Protease 28-IN-4
  • Ubiquitin-specific Protease 28 Inhibitor 4
  • USP28 Inhibitor 4
Molecular Formula
C22H18Cl2N2O3S
Formula Weight
461.4
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: Sparingly soluble: 1-10 mg/ml
SMILES
CS(=O)(C1=CC(Cl)=C(C(NC2=CC=C(Cl)C(C3=CC4=C(C=C3)CNC4)=C2)=O)C=C1)=O
InChi Code
InChI=1S/C22H18Cl2N2O3S/c1-30(28,29)17-5-6-18(21(24)10-17)22(27)26-16-4-7-20(23)19(9-16)13-2-3-14-11-25-12-15(14)8-13/h2-10,25H,11-12H2,1H3,(H,26,27)
InChi Key
FZNJOQKABIINMG-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $39

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    5 mg

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    10 mg

    $270

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    25 mg

    $530

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    Product Description

    USP28-IN-4 is an inhibitor of ubiquitin-specific protease 28 (USP28; IC50 = 0.04 µM).1 It is selective for USP28 over USP2, USP7, USP8, USP9x, ubiquitin C-terminal hydrolase L3 (UCH-L3), and UCH-L5 at 5 µM but does inhibit USP25 at the same concentration. USP28-IN-4 reduces the levels of the USP28 substrate c-Myc in HCT116 and LS 174T colorectal cancer cells when used at concentrations of 80 and 60 µM, respectively, and reduces the viability of the same cells (IC50s = 38.11 and 24.3 µM, respectively). It also reduces the levels of the USP25 substrate tankyrase 1/2 in HCT116 and LS 174T cells.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zhou, D., Xu, Z., Huang, Y., et al. Structure-based discovery of potent USP28 inhibitors derived from Vismodegib. Eur. J. Med. Chem. 254, 115369 (2023).