DUB-IN-1

DUB-IN-1 is an inhibitor of ubiquitin-specific protease 8 (USP8; IC₅₀ = 0.85 µM).¹ It is selective for USP8 over USP7 (IC₅₀ = >100 µM). It inhibits the proliferation of LN-229, U87MG, and T98G glioblastoma cells when used at concentrations ranging from 200 to 800 nM.² DUB-IN-1 (500 and 800 nM) reduces the migration and stemness of LN-229 and T98G cells and sensitizes both to ionizing radiation. It also reduces the viability of a variety of esophageal squamous cell carcinoma (ESCC) cells (IC₅₀s = 1.58-2.14 µM) and induces apoptosis, autophagy, and cell cycle arrest at the G₂/M phase in KYSE-450 and KYSE-30 ESCC cells.³

WARNING This product is not for human or veterinary use.

1. Colombo, M., Vallese, S., Peretto, I., et al. Synthesis and biological evaluation of 9-oxo-9H-indeno[1,2-b]pyrazine-2,3-dicarbonitrile analogues as potential inhibitors of deubiquitinating enzymes. ChemMedChem 5(4), 552-558 (2010).

2. Long, Y., Hu, Z., Yang, D., et al. Pharmacological inhibition of the ubiquitin-specific protease 8 effectively suppresses glioblastoma cell growth. Open Life Sci. 18(1), 20220562 (2023).

3. Sha, B., Sun, Y., Zhao, S., et al. USP8 inhibitor-induced DNA damage activates cell cycle arrest, apoptosis, and autophagy in esophageal squamous cell carcinoma. Cell Biol. Toxicol. 39(5), 2011-2032 (2023).