Host: Insect cells • AA: 1-346 (full length) • Tag: N-terminal GST • MW: 65.6 kDa
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Cdk7 (human, recombinant)

Item No. 42000

Product Insert (PDF)
Technical Information
Synonyms
  • CAK1
  • CDK-activating Kinase 1
  • Cell Division Protein Kinase 7
  • Cyclin-dependent Kinase 7
Source
Recombinant human N-terminal GST-tagged Cdk7 expressed in insect cells
Amino Acids
1-346 (full length)
MW
65.6 kDa
Formulation (Request formulation change)
50 mM Tris-HCl, pH 7.5, 200 mM sodium chloride, and 20% glycerol
UniProt Accession №
P50613
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µg

    $1,296

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    500 µg

    $4,553

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    Product Description

    Cyclin-dependent kinase 7 (Cdk7) is a CDK-activating kinase (CAK) that is mainly involved in cell cycle progression and gene transcription, but also has a role DNA repair.1,2 It is composed of an N-terminal domain that binds cyclin H and a C-terminal domain that binds the CAK assembly factor MAT1 and putative nuclear localization sequences at the N- and C-termini.2 Cdk7 is ubiquitously expressed and localizes to the nucleus.3 In the context of cell cycle progression, Cdk7, in the CAK complex with cyclin H and MAT1, phosphorylates other CDKs such as Cdk1/cyclin A and Cdk2/cyclin E to activate the cell cycle.2 Cdk7 is also a component of the transcription factor IIH (TFIIH) complex where it activates RNA polymerase II directly via phosphorylation, which influences transcription initiation, or indirectly through activation of Cdk9, which promotes elongation. Knockout of CDK7 or inhibition of Cdk7 inhibits breast cancer proliferation in vitro and tumor growth in vivo.4 The intratumoral expression of CDK7 is increased in patients with oral squamous cell carcinoma (OSCC) and is associated with increased tumor grade and poor prognosis.5 Cayman’s Cdk7 (human, recombinant) protein has a calculated molecular weight of 65.6 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Diab, S., Yu, M., and Wang, S. CDK7 inhibitors in cancer therapy: The sweet smell of success? J. Med. Chem. 63(14), 7458-7474 (2020).

    2. Gong, Y., and Li, H. CDK7 in breast cancer: Mechanisms of action and therapeutic potential. Cell Commun. Signal. 22(1), 226 (2024).

    3. Bartkova, J., Zemanova, M., and Bartek, J. Expression of CDK7/CAK in normal and tumour cells of diverse histogenesis, cell-cycle position and differentiation. Int. J. Cancer 66, 732-737 (1996).

    4. Wang, Y., Zhang, T., Kwiatkowski, N., et al. CDK7-dependent transcriptional addiction in triple-negative breast cancer. Cell 163(1), 174-186 (2015).

    5. Jiang, L., Huang, R., Wu, Y., et al. Overexpression of CDK7 is associated with unfavourable prognosis in oral squamous cell carcinoma. Pathology 51(1), 74-80 (2019).