Active • Host: Insect cells • AA: 1-297 • MW: 33.2 kDa
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

HPK1 (human, recombinant)

Item No. 42014

Product Insert (PDF)
Technical Information
Synonyms
  • MAP4K1
  • MAPK/ERK Kinase Kinase 1
  • MEK Kinase Kinase 1
  • MEKKK 1
  • Mitogen-activated Protein Kinase Kinase Kinase Kinase 1
Endotoxin Testing
<1.0 EU/µg determined by the LAL endotoxin assay
Source
Active recombinant human HPK1 expressed in insect cells
Amino Acids
1-297
MW
33.2 kDa
Formulation (Request formulation change)
50 mM HEPES, pH 7.0, with 500 mM sodium chloride, and 5% glycerol
Host
Insect cells
UniProt Accession №
Q92918
Shipping & Storage Information
Shipping
in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $2,008

    Add

    500 µg

    $7,225

    Add
    Kinase Resource Center
    Discover Products & Resources for Kinase Research
    • Kinase inhibitors, screening libraries, assay kits, & more
    • Tools to study kinase signaling pathways:
      • Growth factor signaling
      • PI3K/Akt/mTOR
      • MAPKs (ERK, p38, & JNK)
      • JAK/STAT signaling
    • Articles, resources, & advice
    EXPLORE NOW
    Product Description

    Hematopoietic progenitor kinase 1 (HPK1) is a Ste20-like protein serine/threonine kinase and member of the MAP4K family with roles in cell signaling and immune regulation.1,2,3 It is composed of an N-terminal kinase domain, a central region that contains four proline-rich sequences, a caspase cleavage site, and a C-terminal citron homology domain.1,2 HPK1 is expressed in hematopoietic cells and localizes to the cytosol. Phosphorylation and activation of HPK1 are dependent on adaptor proteins that link HPK1 to cell surface receptors, such as EGFR, and downstream gene transcription targets, including AP-1, NFAT, and IL-2, to facilitate cell signaling.2 Low expression of MAP4K1, the gene encoding HPK1, is associated with prolonged survival in patients with renal clear cell carcinoma and low-grade glioma.3 MAP4K1 expression is decreased in CD4+ T cells isolated from patients with systemic lupus erythematosus (SLE).4 Cayman’s HPK1 (human, recombinant) protein can be used for enzyme activity assays. This protein has a calculated molecular weight of 33.2 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zhou, L., Wang, T., Zhang, K., et al. The development of small-molecule inhibitors targeting HPK1. Eur. J. Med. Chem. 244, 114819 (2022).

    2. Boomer, J.S., and Tan, T.-H. Functional interactions of HPK1 with adaptor proteins. J Cell Biochem 95(1), 34-44 (2005).

    3. Si, J., Shi, X., Sun, S., et al. Hematopoietic progenitor kinase1 (HPK1) mediates t cell dysfunction and is a druggable target for T cell-based immunotherapies. Cancer Cell 38(4), 551-566 (2020).

    4. Chuang, H.-C., and Tan, T.-H. MAP4K family kinases and DUSP family phosphatases in T-cell signaling and systemic lupus erythematosus. Cells 8(11), 1433 (2019).