IRAK4 (human, recombinant)

IL-1 receptor-associated kinase 4 (IRAK4) is a serine/threonine kinase involved in Toll/IL-1 receptor (TIR) signaling.^1,2 It is composed of an N-terminal death domain, which is involved in dimerization and interaction with MyD88, a proline/serine/threonine-rich domain, and a C-terminal kinase domain.² IRAK4 is ubiquitously expressed and localizes to the cytoplasm.^1,3 It has roles in innate and adaptive immunity, interacts with IRAK1, MyD88, and TNF receptor-associated factor 6 (TRAF6), and signals through the NF-κB and MAPK pathways.^2,1 Homozygous knockout of Irak4 increases susceptibility to M. bovis infection in mice and decreases survival and increases lung bacterial burden in a mouse model of M. tuberculosis infection, however, Irak4^-/- mice are resistant to LPS-induced septic shock.^4,5 Mutations in IRAK4 are associated with an increased risk of invasive bacterial infections in children.⁶ Cayman’s IRAK4 (human, recombinant) protein can be used for enzyme activity assays. This protein has a calculated molecular weight of 34.4 kDa.

WARNING This product is not for human or veterinary use.

1. Li, S., Strelow, A., Fontana, E.J., et al. IRAK-4: A novel member of the IRAK family with the properties of an IRAK-kinase. Proc. Natl. Acad. Sci. USA 99(8), 5567-5572 (2002).

2. Su, L.-C., Xu, W.-D., and Huang, A.-F. IRAK family in inflammatory autoimmune diseases. Autoimmun. Rev. 19(3), 102461 (2020).

3. Nagpal, K., Plantinga, T.S., Sirois, C.M., et al. Natural loss-of-function mutation of myeloid differentiation protein 88 disrupts its ability to form Myddosomes. The Journal of Biological Chemisty 286(13), 11875-11882 (2011).

4. Marinho, F.V., Fahel, J.S., Scanga, C.A., et al. Lack of IL-1 receptor–associated kinase-4 leads to defective Th1 cell responses and renders mice susceptible to mycobacterial infection. J. Immunol. 197(5), 1852-1863 (2016).

5. Suzuki, N., Suzuki, S., Duncan, G.S., et al. Severe impairment of interleukin-1 and toll-like receptor signalling in mice lacking IRAK-4. Nature 416(6882), 750-756 (2002).

6. Ku, C.-L., von Bernuth, H., Picard, C., et al. Selective predisposition to bacterial infections in IRAK-4-deficient children: IRAK-4-dependent TLRs are otherwise redundant in protective immunity. J. Exp. Med. 204(10), 2407-2422 (2007).