Host: E. coli • AA: 30-390 • Tag: N-terminal His • MW: 47 kDa
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PDHE1-A (human, recombinant)

Item No. 42229

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Pyruvate Dehydrogenase E1
  • Pyruvate Dehydrogenase E1 Component Subunit α
Purity
≥70% estimated by SDS-PAGE
Source
Recombinant human N-terminal His-tagged PDHE1-A expressed in E. coli
Amino Acids
30-390
MW
47 kDa
Formulation (Request formulation change)
50 mM sodium phosphate, pH 7.0, with 300 mM sodium chloride, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, and 25% glycerol
UniProt Accession №
P08559
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    50 µg

    $193

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    Cayman Chemical
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    Product Description

    Pyruvate dehydrogenase E1 component subunit α (PDHE1-A), also known as pyruvate dehydrogenase E1, is a dehydrogenase and member of the 2-oxo acid dehydrogenase family.1,2 It is a tetramer composed of a cleavable mitochondrial signaling sequence and two α- and β domains with active sites between each of the α/β domain interfaces.2 It is a member of the mitochondrial pyruvate dehydrogenase complex and catalyzes the oxidative decarboxylation of pyruvate, which is the first reaction in the enzymatic conversion of pyruvate to acetyl-CoA. Phosphorylation of serine 203, -264, or -271 inhibits PDHE1-A enzymatic activity by blocking active site associations with its cofactor thiamine pyrophosphate (Item No. 20254) and serves as a reversible regulatory mechanism for PDHE1-A and pyruvate dehydrogenase complex activity.3 Dichloroacetate-induced phosphorylation of PDHE1-A reduces the viability of HSC-2 and HSC-3 oral squamous cell carcinoma cells.4 Mutations in PDHA1, the gene encoding PDHE1-A, are associated with lactic acidosis, developmental delay, cerebral atrophy, seizures, and elevated urine and cerebrospinal fluid (CSF) levels of pyruvate in patients with the inborn error of metabolism pyruvate dehydrogenase complex deficiency (PDCD).5,6 Cayman's PDHE1-A (human, recombinant) protein can be used or enzyme activity assays. This protein has a calculated molecular weight of 47 kDA.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Milne, J.L.S. Structure and regulation of pyruvate dehydrogenases. Encyclopedia of Biological Chemistry 321-328 (2013).

    2. Ciszak, E.M., Korotchkina, L.G., Dominiak, P.M., et al. Structural basis for flip-flop action of thiamin pyrophosphate-dependent enzymes revealed by human pyruvate dehydrogenase. The Journal of Biological Chemisty 278(23), 21240-21246 (2003).

    3. Korotchkina, L.G., and Patel, M.S. Probing the mechanism of inactivation of human pyruvate dehydrogenase by phosphorylation of three sites. The Journal of Biological Chemisty 276(8), 5731-5738 (2001).

    4. Ruggieri, V., Agriesti, F., Scrima, R., et al. Dichloroacetate, a selective mitochondria-targeting drug for oral squamous cell carcinoma: A metabolic perspective of treatment. Oncotarget 6(2), 1217-1230 (2015).

    5. Patel, K.P., O'Brien, T.W., Subramony, S.H., et al. The spectrum of pyruvate dehydrogenase complex deficiency: Clinical, biochemical and genetic features in 371 patients. Mol. Genet. Metab. 105(1), 34-43 (2012).

    6. Schillaci, L.-A.P., DeBrosse, S.D., and McCandless, S.E. Inborn errors of metabolism with acidosis: Organic acidemias and defects of pyruvate and ketone body metabolism. Pediatr. Clin. North Am. 65(2), 209-230 (2018).