Active • Host: Insect cells (Sf9) • AA: 513-827 • Tag: C-terminal His • MW: 39 kDa
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JAK2 JH2 Domain (human, recombinant; aa 513-827) - Biotinylated

Item No. 42273

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Janus-Associated Kinase 2
  • JTK10
  • Tyrosine-Protein Kinase JAK2
Purity
≥90% estimated by SDS-PAGE
Source
Recombinant human C-terminal His-tagged biotinylated JAK2 JH2 domain expressed in insect cells (Sf9)
Amino Acids
513-827
MW
39 kDa
Formulation (Request formulation change)
40 mM Tris-HCl, pH 8.0, 300 mM sodium chloride, 2.2 mM potassium chloride, 20% glycerol, and 0.2 mM TCEP
UniProt Accession №
O60674
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    10 µg

    $413

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    Product Description

    JAK2 is a non-receptor tyrosine kinase that has roles in immune signaling.1,2 It is composed of N-terminal FERM and SH2 domains, a regulatory JH2 pseudokinase domain, and a C-terminal JH1 kinase domain. It is widely expressed and associates with class I and class II cytokine receptors at the plasma membrane.3,4 Activation of these cytokine receptors activates JAK2 and induces its dimerization and kinase activity, leading to JAK2 phosphorylation of STAT transcription factors and transcription of immune-related target genes. JAK2 signaling is inhibited by the suppressor of cytokine signaling (SOCS) proteins SOCS1 and SOCS3.5,6 Gain-of-function mutations in JAK2, such as JAK2V617F in the JH2 domain, are associated with various blood disorders, including leukemias and myeloproliferative neoplasms.4 Cayman’s JAK2 JH2 Domain (human, recombinant; aa 513-827) - Biotinylated protein can be used for pull-down assays and has a calculated molecular weight of 39 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Leonard, W.J., and O'Shea, J.J. Jaks and STATs: Biological implications. Annu. Rev. Immunol. 16, 293-322 (1998).

    2. Haan, C., Kreis, S., Margue, C., et al. Jaks and cytokine receptors—an intimate relationship. Biochem. Pharmacol. 72(11), 1538-1546 (2006).

    3. Parganas, E., Wang, D., Stravopodis, D., et al. Jak2 is essential for signaling through a variety of cytokine receptors. Cell 93(3), 385-395 (1998).

    4. Hubbard, S.R. Mechanistic insights into regulation of JAK2 tyrosine kinase. Front. Endocrinol. (Lausanne) 8, 361 (2018).

    5. Kile, B.T., and Alexander, W.S. The suppressors of cytokine signalling (SOCS). Cell. Mol. Life Sci. 58(11), 1627-1635 (2001).

    6. Yoshimura, A., and Yasukawa, H. JAK’s SOCS: A mechanism of inhibition. Immunity 36(2), 157-159 (2012).