A quenched fluorescent probe for GST activity
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DNs-Rh

Item No. 42354

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
9-(2-carboxyphenyl)-3,6-bis[[(2,4-dinitrophenyl)sulfonyl]amino]-xanthylium, inner salt
CAS Number
1028386-82-4
Synonyms
  • bis-DNs-Rh
Molecular Formula
C32H18N6O15S2
Formula Weight
790.6
Purity
≥95%
Emission
520 nm
Excitation
496 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: soluble
SMILES
O=S(C(C([N+]([O-])=O)=C1)=CC=C1[N+]([O-])=O)(NC2=CC([O+]=C3C(C=CC(NS(C(C([N+]([O-])=O)=C4)=CC=C4[N+]([O-])=O)(=O)=O)=C3)=C5C6=C(C([O-])=O)C=CC=C6)=C5C=C2)=O
InChi Code
InChI=1S/C32H18N6O15S2/c39-32(40)22-4-2-1-3-21(22)31-23-9-5-17(33-54(49,50)29-11-7-19(35(41)42)15-25(29)37(45)46)13-27(23)53-28-14-18(6-10-24(28)31)34-55(51,52)30-12-8-20(36(43)44)16-26(30)38(47)48/h1-16,33-34H
InChi Key
BBQPPVIVVGPYHO-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    0.1 µmol

    $549

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    Product Description

    DNs-Rh is a cell-permeable quenched fluorescent probe for the detection of pan-glutathione S-transferase (GST) activity in adherent or suspension cells, cell or tissue lysates, or purified enzyme preparations. It is composed of rhodamine 110 (Item No. 19061) protected by the GST substrate dinitrobenzenesulfonamide (DNB).1,2 Upon deprotection by GSTs, rhodamine 110 is released, and its fluorescence can be quantified using excitation/emission maxima of 496/520 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Shibata, A., Furukawa, K., Abe, H., et al. Rhodamine-based fluorogenic probe for imaging biological thiol. Bioorg. Med. Chem. Lett. 18(7), 2246-2249 (2008).

    2. Ålander, J., Johansson, K., Heuser, V.D., et al. Characterization of a new fluorogenic substrate for microsomal glutathione transferase 1. Anal. Biochem. 390(1), 52-56 (2009).