For immunochemical detection of FAK1
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FAK1 Rabbit Monoclonal Antibody (Clone RM472)

Item No. 42837

Product Insert (PDF)
Technical Information
Synonyms
  • FADK1
  • Focal Adhesion Kinase 1
  • p125FAK
  • pp125FAK
  • Protein-Tyrosine Kinase 2
  • PTK2
Immunogen
A peptide corresponding to residues surrounding Tyr397 of human FAK1
Clone Designation
RM472
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) FAK1
Species Reactivity
(+) Human(+) Mouse(+) Rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

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    Cayman Chemical
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    Product Description

    Focal adhesion kinase 1 (FAK1) is a non-receptor tyrosine kinase and adaptor protein.1,2 It is composed of an N-terminal FERM domain, a proline-rich region, a catalytic domain, two additional proline-rich regions, and a C-terminal focal adhesion-targeting (FAT) domain, which can also be autonomously expressed as FAK-related nonkinase (FRNK) and act as an inhibitor of FAK activity.3,4 FAK is ubiquitously expressed and localizes to focal adhesion sites at the plasma membrane, as well as the cytoplasm and nucleus.2,3,5 It is activated by autophosphorylation of Tyr397, which recruits c-Src to initiate signaling through PI3K/Akt or MAPK/ERK pathways, and is involved in integrin signaling, cell adhesion, migration, and survival.1,2 Overexpression of FAK is associated with various cancers and metastasis.6,1 Cayman’s FAK1 Rabbit Monoclonal Antibody (Clone RM472) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Katoh, K. Signal transduction mechanisms of focal adhesions: Src and FAK-mediated cell response. Front. Biosci. (Landmark Ed.) 29(11), 392 (2024).

    2. Mitra, S.K., Hanson, D.A., and Schlaepfer, D.D. Focal adhesion kinase: In command and control of cell motility. Nat. Rev. Mol. Cell Biol. 6(1), 56-68 (2005).

    3. Samarel, A.M. Focal adhesion signaling in heart failure. Pflugers Arch. 466(6), 1101-1111 (2014).

    4. Koshman, Y., Kim, T., Chu, M., et al. FRNK inhibition of focal adhesion kinase-dependent signaling and migration in vascular smooth muscle cells. Arterioscler. Thromb. Vasc. Biol. 30(11), 2226-2233 (2010).

    5. Golubovskaya, V.M., Finch, R., and Cance, W.G. Direct interaction of the N-terminal domain of focal adhesion kinase with the N-terminal transactivation domain of p53. The Journal of Biological Chemisty 280(26), 25008-25021 (2005).

    6. Parsons, J.T., Slack-Davis, J., Tilghman, R., et al. Focal adhesion kinase: Targeting adhesion signaling pathways for therapeutic intervention. Clin. Cancer Res. 14(3), 627-632 (2008).