For immunochemical detection of 5-hmC
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5-Hydroxymethylcytosine DNA Matched Antibody Pair (Clones RM236/RM476)

Item No. 42874

Product Insert (PDF)
Technical Information
Synonyms
  • 5-hmC
Clone Designation
RM236/RM476
Formulation (Request formulation change)
Protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol and 0.09% sodium azide and PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Applications
Sandwich ELISA, other sandwich assays
Cross Reactivity
(+) 5-hmC(-) Cytosine(-) 5-mC(-) 5-caC(-) 5-fC
Species Reactivity
(+) Species-independent
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea

    $702

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    Product Description

    5-Hydroxymethylcytosine (5-hmC) is an epigenetic modification formed from the oxidation of 5-methylcytosine (5-mC) by Tet dioxygenases.1 5-hmC is primarily a stable DNA modification, but it can be oxidized by Tet enzymes and its products further modified to generate nonmethylated cytosine, indicating a role as an intermediate in DNA demethylation as well.2,3,4 It is associated with actively transcribed genes and recognized by a variety of proteins, including proteins involved in DNA repair and DNA metabolic processes.2,4 5-hmC has been found in all mammalian tissues but levels are higher in the brain relative to other tissues.5 The percentage of genomic 5-hmC in mouse cerebellum increases during postnatal development until adulthood, and genes acquiring intragenic 5-hmC are enriched in pathways associated with age-related neurodegenerative disease pathways in adult mice.6 In contrast, 5-hmC levels are reduced by up to 8-fold in cancer tissues.2,7 Cayman’s 5-Hydroxymethylcytosine DNA Matched Antibody Pair (Clones RM236/RM476) contains 5-Hydroxymethylcytosine Rabbit Monoclonal Antibody (Clone RM236) (Item No. 20723) as a capture antibody and Adenine-Guanine Rabbit Monoclonal Antibody - Biotinylated (Clone RM476) as a detection antibody. This antibody pair can be used for sandwich ELISAs or other sandwich assays for the specific detection of 5-hmC in ssDNA.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Tahiliani, M., Koh, K.P., Shen, Y., et al. Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1. Science 324(5929), 930-935 (2009).

    2. Bachman, M., Uribe-Lewis, S., Yang, X., et al. 5-Hydroxymethylcytosine is a predominantly stable DNA modification. Nat. Chem. 6(12), 1049-1055 (2014).

    3. Breiling, A., and Lyko, F. Epigenetic regulatory functions of DNA modifications: 5-Methylcytosine and beyond. Epigenetics Chromatin 8, 24 (2015).

    4. Spruijt, C.G., Gnerlich, F., Smits, A.H., et al. Dynamic readers for 5-(hydroxy)methylcytosine and its oxidized derivatives. Cell 152(5), 1146-1159 (2013).

    5. Globisch, D., Münzel, M., Müller, M., et al. Tissue distribution of 5-hydroxymethylcytosine and search for active demethylation intermediates. PLoS One 5(12), e15367 (2010).

    6. Song, C.X., Szulwach, K.E., Fu, Y., et al. Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine. Nat. Biotechnol. 29(1), 68-72 (2011).

    7. Wang, Z., Du, M., Yuan, Q., et al. Epigenomic analysis of 5-hydroxymethylcytosine (5hmC) reveals novel DNA methylation markers for lung cancers. Neoplasia 22(3), 154-161 (2020).