IgG3 Antibody Pair (Clones RM119/RM129)

Immunoglobulin G (IgG) is a member of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response.¹ It is produced by B cells and later secreted by plasma cells and is the most abundant circulating antibody in human and mouse serum.^1,2,3 IgG consists of two heavy chains of approximately 50 kDa each and two light chains of approximately 25 kDa each.¹ The heavy chains are linked together by disulfide bonds to form an Fc region and also combine with the light chains to form the Fab region, which mediate receptor and antigen binding, respectively.⁴ IgG exists as four isotypes in humans, IgG1, IgG2, IgG3, and IgG4, each of which has a distinct effector function. IgG3 production is driven by bacterial- or viral-associated antigens, including HIV-1 and Staphylococcus antigens, and occurs early in the immune response following IgM class-switching.^2,5 IgG3 binds to and neutralizes pathogens, as well as activates complement and opsonizes bacteria, leading to complement-dependent cytotoxicity (CDC) and antibody-dependent cell-mediated cytotoxicity (ADCC), respectively. Serum IgG3 levels are increased in patients with primary biliary cirrhosis, Sjögren’s syndrome, systemic sclerosis, or systemic lupus erythematosus (SLE).⁶ Cayman's IgG3 (human) Matched Antibody Pair (Clones RM119/RM129) contains IgG3 (human) Rabbit Monoclonal Antibody (Clone RM119) (Item No. 32122) as a capture antibody, Ig Light Chain (human) Rabbit Monoclonal Antibody - Biotinylated (Clone RM129) (Item No. 32112) as a detection antibody, and a sample diluent to reduce non-specific background. The antibody pair can be used for sandwich ELISAs and other sandwich assays for the specific detection of IgG3 in human samples and experimentally introduced human IgG3 in monkey, mouse, or rat serum.

WARNING This product is not for human or veterinary use.

1.  .

2.  .

3.  .

4.  .

5.  .

6.  .