Human Fibroblast Growth Factor Receptor 3 Assay for Paracrine FGF Signaling

The Fibroblast Growth Factors (FGFs) are broad-spectrum mitogens that, through their receptor interactions, regulate a variety of cellular functions including migration, proliferation, differentiation, metabolism and survival. In particular, FGF/FGFR signaling plays a critical role in regulating metabolism in the kidney, liver, brain, intestine and adipose tissues. Not surprisingly, dysfunctional FGFR signaling can lead to a range of physiological disorders. For example, mutation, amplification, and gene fusion may result in abnormal morphogenesis and the progression of several types of cancer. Consequently, the FGF receptors continue to command much interest as targets for drug development and drug safety screening. INDIGO's FGFR3 for Paracrine FGF Signaling Reporter Cells contain the luciferase reporter gene functionally linked to tandem NFAT consensus response element sequences upstream of a minimal promoter. Activated NFAT binds to these response elements to initiate the formation of a complete transcription complex that drives Luc gene expression. Quantifying relative changes in luciferase activity in the treated reporter cells relative to the untreated cells provides a sensitive, dose-dependent surrogate measure of drug-induced changes in FGFR3 activity. Accordingly, the principal application of this reporter assay is in the screening of test materials to quantify any functional interactions, either activating or inhibitory, that they may exert against FGFR3, or the coupled Ca⁺²∙calcineurin/NFAT signal transduction pathway. To reiterate, this assay is primarily focused on the paracrine signaling activities of FGFR3/FGFs.

Reporter Cells are transiently transfected and prepared as frozen stocks using INDIGO's proprietary CryoMite™ process. This cryo-preservation method allows for the immediate dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, or cell titer adjustments prior to assay setup. [INDIGO Catalog Item Nos. IB42001, IB42001-32]

WARNING This product is not for human or veterinary use.