Host: Insect cells • AA: 1-427 (full length) • Tag: N-terminal GST • MW: 75 kDa
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JNK1 α2 Isoform (human, recombinant)

Item No. 43698

Product Insert (PDF)
Technical Information
Synonyms
  • c-Jun N-terminal Kinase 1
  • JNK-46
  • MAPK8
  • MAP Kinase 8
  • Mitogen-activated Protein Kinase 8
  • SAPK1C
  • Stress-activated Protein Kinase 1C
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/µg determined by the LAL endotoxin assay
Source
Recombinant human N-terminal GST-tagged JNK1 α2 isoform expressed in insect cells
Amino Acids
1-427 (full length)
MW
76 kDa
Formulation (Request formulation change)
Sterile 50 mM Tris, with 100 mM sodium chloride, pH 8.0, and 25% glycerol
Host
Insect cells
UniProt Accession №
P45983
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    50 µg

    $218

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    Product Description

    JNK1 is a stress-activated serine/threonine protein kinase and member of the MAPK family.1 It is composed of a short N-terminal helical domain, a flexible connector that contains the active site, and a large helical C-terminal domain.2 JNK1 is encoded by MAPK8, which produces four isoforms via alternative splicing.3 JNK1 is ubiquitously expressed and is found in the cytoplasm.1,3,4 JNKs are activated by phosphorylation at threonine 183 (Thr183) and tyrosine 185 (Tyr185) by MAP kinase kinase 7 (MKK7) and MKK4, respectively, in response to stress stimuli, such as cellular stress, radiation, inflammation, or oxidative stress.5,1 After activation, JNKs localize to the nucleus where they phosphorylate transcription factors, nuclear receptors, and adaptor proteins that broadly regulate cell proliferation, cell survival, and apoptosis.4 Increased intratumoral levels of JNK1 are associated with decreased survival in patients with hepatocellular carcinoma (HCC).6 Cayman's JNK1 α2 Isoform (human, recombinant) protein consists of 652 amino acids and has a calculated molecular weight of 75 kDa. This protein migrates at approximately 65 kDa by SDS-PAGE under reducing conditions.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kumar, A., Singh, U.K., Kini, S.G., et al. JNK pathway signaling: A novel and smarter therapeutic target for various biological diseases. Future Med. Chem. 7(15), 2065-2086 (2015).

    2. Heo, Y.S., Kim, S.K., Seo, C.I., et al. Structural basis for the selective inhibition of JNK1 by the scaffolding protein JIP1 and SP600125. EMBO J. 23(11), 2185-2195 (2004).

    3. Zhang, T., Inesta-Vaquero, F., Niepel, M., et al. Discovery of potent and selective covalent inhibitors of JNK. Chem. Biol. 19(1), 140-154 (2012).

    4. Bogoyevitch, M.A., and Kobe, B. Uses for JNK: The many and varied substrates of the c-Jun N-terminal kinases. Microbiol. Mol. Biol. Rev. 70(4), 1061-1095 (2006).

    5. Thévenin, A.F., Zony, C.L., Bahnson, B.J., et al. Activation by phosphorylation and purification of human c-Jun N-terminal kinase (JNK) isoforms in milligram amounts. Protein Expr. Purif. 75(2), 138-146 (2011).

    6. Chang, Q., Chen, J., Beezhold, K.J., et al. JNK1 activation predicts the prognostic outcome of the human hepatocellular carcinoma. Mol. Cancer 8, 64 (2009).