Active • Host: Insect cells • AA: 1-476 (full length) • Tag: N-terminal His • MW: 59 kDa
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Chk1 (human, recombinant)

Item No. 43751

Product Insert (PDF)
Technical Information
Synonyms
  • Cell Cycle Checkpoint Kinase 1
  • Checkpoint Kinase 1
  • CHK1 Checkpoint Homolog
  • Serine/threonine-protein Kinase Chk1
Purity
≥80% estimated by SDS-PAGE
Source
Active recombinant human N-terminal His-tagged Chk1 expressed in insect cells
Amino Acids
1-476 (full length)
MW
59 kDa
Formulation (Request formulation change)
Sterile 50 mM sodium phosphate, pH 7.5, with 300 mM sodium chloride, 50-200 mM imidazole, 0-0.1 mM PMSF, 0.2-1.0 mM DTT, and 10-25% glycerol
UniProt Accession №
O14757
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    10 µg

    $383

    Add

    50 µg

    $1,245

    Add

    500 µg

    $2,871

    Add
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    Product Description

    Checkpoint kinase 1 (Chk1) is a serine/threonine kinase and downstream effector of ataxia-telangiectasia and RAD3-related protein/kinase (ATR) in the cellular DNA damage response.1,2 It is composed of an N-terminal kinase domain, a flexible linker region, a serine/glutamine cluster, and a C-terminal regulatory domain, which contains two conserved motifs, CM1 and CM2, that mediate kinase autoinhibition.2,3 Chk1 is ubiquitously expressed and localized primarily to the nucleus.3,4 In response to single-stranded DNA (ssDNA) generated from replication stress, DNA crosslinking, or other damage, ATR phosphorylates Chk1 at serine 345 and 317 inducing its activation and cell cycle arrest at the S or G2/M phase.4 Loss of one copy of Chek1 induces erythropoietic defects, anemia, and sudden death in mice, while Chek1 knockout is embryonically lethal. Pharmacologic inhibition of Chk1 increases DNA damage and enhances antitumor efficacy of chemotherapeutic agents in various mouse xenograft models.5 Cayman’s Chk1 (human, recombinant) protein can be used for enzyme activity assay and Western blot (WB) applications. This protein has a calculated molecular weight of 59 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Qiu, Z., Oleinick, N.L., and Zhang, J. ATR/CHK1 inhibitors and cancer therapy. Radiother. Oncol. 126(3), 450-464 (2018).

    2. Zhang, Y., and Hunter, T. Roles of Chk1 in cell biology and cancer therapy. Int. J. Cancer 134(5), 1013-1023 (2014).

    3. Day, M., Parry-Morris, S., Houghton-Gisby, J., et al. Structural basis for recruitment of the CHK1 DNA damage kinase by the CLASPIN scaffold protein. Structure 29(6), 531-539 (2021).

    4. Neizer-Ashun, F., and Bhattacharya, R. Reality CHEK: Understanding the biology and clinical potential of CHK1. Cancer Lett. 497, 202-211 (2021).

    5. Barnard, D., Diaz, H.B., Burke, T., et al. LY2603618, a selective CHK1 inhibitor, enhances the anti-tumor effect of gemcitabine in xenograft tumor models. Invest New Drugs 34(1), 49-60 (2016).