Active • Host: Insect cells • AA: 1-543 (full length) • Tag: N-terminal GST • MW: 88 kDa
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Chk2 (human, recombinant)

Item No. 43753

Product Insert (PDF)
Technical Information
Synonyms
  • Cell Cycle Checkpoint Kinase 2
  • Checkpoint Kinase 2
  • CHK2 Checkpoint Homolog
  • Serine/threonine-protein Kinase Chk2
Purity
≥90% estimated by SDS-PAGE
Source
Active recombinant human N-terminal GST-tagged Chk2 expressed in insect cells
Amino Acids
1-543 (full length)
MW
88 kDa
Formulation (Request formulation change)
50 mM Tris-HCl, pH 7.5, with 50-300 mM sodium chloride, 10 mM glutathione, 0.1 mM EDTA, 0.25-1.0 mM DTT, 0-0.1 mM PMSF, and 10-25% glycerol
UniProt Accession №
O96017
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    10 µg

    $383

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    50 µg

    $1,245

    Add
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    Product Description

    Checkpoint kinase 2 (Chk2) is a serine/threonine kinase and downstream effector of ataxia-telangiectasia mutated kinase (ATM) in the cellular DNA damage response.1,2 It is composed of an N-terminal serine-glutamine/threonine-glutamine cluster domain (SCD), a central forkhead-associated (FHA) domain, and a C-terminal serine/threonine kinase domain.2 Chk2 is ubiquitously expressed and localized to the nucleus. In response to double stranded breaks (DSBs) generated by genotoxins or radiation, Chk2 is phosphorylated and activated by ATM, inducing cell cycle arrest at the S or G2/M phase.1 Chk2 also has roles in various mitotic processes, including spindle formation, cytokinesis, checkpoint signaling, and chromosome segregation.1,3 Overexpression of Chek2 is associated with disease progression in papillary thyroid cancer.4 Cayman’s Chk2 (human, recombinant) protein can be used for enzyme activity assay and Western blot (WB) applications. This protein has a calculated molecular weight of 88 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Smith, J., Tho, L.M., Xu, N., et al. The ATM-Chk2 and ATR-Chk1 pathways in DNA damage signaling and cancer. Adv. Cancer Res. 108, 73-112 (2010).

    2. Cai, Z., Chehab, N.H., and Pavletich, N.P. Structure and activation mechanism of the CHK2 DNA damage checkpoint kinase. Mol. Cell. 35(6), 818-829 (2009).

    3. Black, E.M., Parrado, C.A.R., Trier, I., et al. Chk2 sustains PLK1 activity in mitosis to ensure proper chromosome segregation. Nat. Commun. 15(1), 10782 (2024).

    4. Zhao, W., Chen, S., Hou, X., et al. CHK2 promotes anoikis and is associated with the progression of papillary thyroid cancer. Cell. Physiol. Biochem. 45(4), 1590-1602 (2018).