Active • Host: Insect cells • AA: 1-441 • Tag: N-terminal GST • MW: 86 kDa
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WNK4 (mouse, recombinant)

Item No. 43754

Product Insert (PDF)
Technical Information
Synonyms
  • Protein Kinase Lysine-deficient 4
  • Protein Kinase With No Lysine 4
  • Serine/threonine Protein Kinase WNK4
  • With No Lysine Kinase 4
  • WNK Lysine Deficient Protein Kinase 4
Purity
≥85% estimated by SDS-PAGE
Source
Active recombinant mouse N-terminal GST-tagged WNK4 expressed in insect cells
Amino Acids
1-441
MW
86 kDa
Formulation (Request formulation change)
Sterile 50 mM Tris-HCl, pH 7.5, 50-300 mM sodium chloride, 10 mM glutathione, 0.1 mM EDTA, 0.25-1 mM DTT, 0-0.1 mM PMSF, and 10-25% glycerol
Host
Insect cells
UniProt Accession №
Q80UE6
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    10 µg

    $383

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    50 µg

    $1,245

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    Product Description

    WNK lysine deficient protein kinase 4 (WNK4) is a kinase member of the serine/threonine protein kinase family.1,2 It is composed of an N-terminal kinase domain, a PF2-like autoinhibitory domain, a coiled-coiled domain, an acidic motif, an RFQV sequence, a second coil-coiled domain, and a C-termina ddl calmodulin-binding domain.1,3 WNK4 localizes to cell tight junctions and is primarily expressed in distal nephron epithelial cells.1 It regulates renal ion exchange by positively regulating kidney-specific Na-K-Cl cotransporter (NKCC2), Na-Cl symporter (NCC), and pendrin and negatively regulating epithelial sodium channel (ENac) and inward-rectifier potassium channel 1.1 (Kir1.1).1,3,4 Missense mutations in WNK4 lead to the development of familial hyperkalemic hypertension (FHHt), a syndrome characterized by hypertension and increased blood levels of potassium and chloride caused by disrupted kidney ion exchange.5 Cayman's WNK4 protein can be used for enzyme activity and Western blot (WB) applications. This protein consists of 441 amino acids and has a calculated molecular weight of 86 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Murillo-de-Ozores, A.R., Rodríguez-Gama, A., Carbajal-Contreras, H., et al. WNK4 kinase: From structure to physiology. Am. J. Physiol. Renal Physiol. 320(3), F378-F403 (2021).

    2. Peng, J.-B., and Warnock, D.G. WNK4-mediated regulation of renal ion transport proteins. Am. J. Physiol. Renal Physiol. 293(4), F961-F973 (2007).

    3. Ring, A.M., Leng, Q., Rinehart, J., et al. An SGK1 site in WNK4 regulates Na+ channel and K+ channel activity and has implications for aldosterone signaling and K+ homeostasis. Proc. Natl. Acad. Sci. USA 104(10), 4025-4029 (2007).

    4. Terker, A.S., Castañeda-Bueno, M., Ferdaus, M.Z., et al. With no lysine kinase 4 modulates sodium potassium 2 chloride cotransporter activity in vivo. Am. J. Physiol. Renal Physiol. 315(4), F781-F790 (2018).

    5. Wilson, F.H., Disse-Nicodème, S., Choate, K.A., et al. Human hypertension caused by mutations in WNK kinases. Science 293(5532), 1107-1112 (2001).